Comparison of the effect of different opsonins on the phagocytosis of fluorescein-labeled staphylococcal bacteria by chicken heterophils
Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and staphylococcal antisera significantly increased bacterial phagocytosis compared to samples without an opsonin. The staphylococcal antisera did...
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| Published in | Avian diseases Vol. 40; no. 4; p. 778 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
01.10.1996
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| Subjects | |
| Online Access | Get more information |
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| Abstract | Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and staphylococcal antisera significantly increased bacterial phagocytosis compared to samples without an opsonin. The staphylococcal antisera did not significantly increase bacterial phagocytosis compared to the normal pooled sera. Opsonization appears to increase bacterial phagocytosis but specific antisera may not increase phagocytosis beyond that caused by pooled normal sera. |
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| AbstractList | Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and staphylococcal antisera significantly increased bacterial phagocytosis compared to samples without an opsonin. The staphylococcal antisera did not significantly increase bacterial phagocytosis compared to the normal pooled sera. Opsonization appears to increase bacterial phagocytosis but specific antisera may not increase phagocytosis beyond that caused by pooled normal sera. |
| Author | Andreasen, Jr, J R Andreasen, C B Oughton, J A Sonn, A E |
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| Snippet | Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and... |
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| SubjectTerms | Animals Antibodies, Bacterial - analysis Antibodies, Bacterial - immunology Chickens - blood Chickens - immunology Chickens - physiology Flow Cytometry - methods Flow Cytometry - veterinary Fluorescence Neutrophils - metabolism Opsonin Proteins - pharmacology Phagocytosis - drug effects Phagocytosis - physiology Staphylococcus - immunology Staphylococcus - metabolism |
| Title | Comparison of the effect of different opsonins on the phagocytosis of fluorescein-labeled staphylococcal bacteria by chicken heterophils |
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