Comparison of the effect of different opsonins on the phagocytosis of fluorescein-labeled staphylococcal bacteria by chicken heterophils

Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and staphylococcal antisera significantly increased bacterial phagocytosis compared to samples without an opsonin. The staphylococcal antisera did...

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Bibliographic Details
Published inAvian diseases Vol. 40; no. 4; p. 778
Main Authors Andreasen, C B, Andreasen, Jr, J R, Sonn, A E, Oughton, J A
Format Journal Article
LanguageEnglish
Published United States 01.10.1996
Subjects
Animals
Antibodies, Bacterial - analysis
Antibodies, Bacterial - immunology
Chickens - blood
Chickens - immunology
Chickens - physiology
Flow Cytometry - methods
Flow Cytometry - veterinary
Fluorescence
Neutrophils - metabolism
Opsonin Proteins - pharmacology
Phagocytosis - drug effects
Phagocytosis - physiology
Staphylococcus - immunology
Staphylococcus - metabolism
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Summary:Heterophil phagocytosis of fluorescein-labeled staphylococcal bacteria was analyzed by flow cytometry. Opsonization with two types of normal pooled sera and staphylococcal antisera significantly increased bacterial phagocytosis compared to samples without an opsonin. The staphylococcal antisera did not significantly increase bacterial phagocytosis compared to the normal pooled sera. Opsonization appears to increase bacterial phagocytosis but specific antisera may not increase phagocytosis beyond that caused by pooled normal sera.
ISSN:0005-2086
DOI:10.2307/1592297