Comparative analysis of mouse and human preimplantation development following POU5F1 CRISPR/Cas9 targeting reveals interspecies differences

• Published in: Human reproduction (Oxford) Vol. 36; no. 5; pp. 1242 - 1252
• Main Authors: Stamatiadis, P, Boel, A, Cosemans, G, Popovic, M, Bekaert, B, Guggilla, R, Tang, M, De Sutter, P, Van Nieuwerburgh, F, Menten, B, Stoop, D, Chuva de Sousa Lopes, S M, Coucke, P, Heindryckx, B
• Format: Journal Article
• Language: English
• Published: England 20.04.2021
• Subjects: genome editing; short palindromic repeats; POU class 5 homeobox 1; CRISPR-associated genes; human embryo; CRISPR-Cas9; mouse embryo; post-implantation development; clustered regularly interspaced; pre-implantation development

What is the role of POU class 5 homeobox 1 (POU5F1) in human preimplantation development and how does it compare with the mouse model? POU5F1 is required for successful development of mouse and human embryos to the blastocyst stage as knockout embryos exhibited a significantly lower blastocyst forma...