Induced expression of melittin, an antimicrobial peptide, inhibits infection by Chlamydia trachomatis and Mycoplasma hominis in a HeLa cell line

As the number of pathogenic microbial strains resistant to different antibiotics increases, amphipathic peptides with antimicrobial activity are promising agents for the therapy of infectious diseases. This work deals with the effect of an amphipathic antimicrobial peptide, melittin, expressed withi...

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Published inInternational journal of antimicrobial agents Vol. 19; no. 2; pp. 133 - 137
Main Authors LAZAREV, V. N, PARFENOVA, T. M, GULARYAN, S. K, MISYURINA, O. Yu, AKOPIAN, T. A, GOVORUN, V. M
Format Journal Article
LanguageRussian
English
Published London Elsevier 01.02.2002
Amsterdam
New York, NY
Subjects
Chlamydia trachomatis - drug effects
Chlamydia trachomatis - physiology
Cytomegalovirus - genetics
Gene Expression Regulation - drug effects
Genetic Vectors - genetics
HeLa Cells
Humans
Melitten - biosynthesis
Melitten - genetics
Melitten - pharmacology
Membrane Potentials
Mycoplasma hominis - drug effects
Mycoplasma hominis - physiology
Promoter Regions, Genetic - genetics
Tetracycline - pharmacology
Transfection
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Abstract As the number of pathogenic microbial strains resistant to different antibiotics increases, amphipathic peptides with antimicrobial activity are promising agents for the therapy of infectious diseases. This work deals with the effect of an amphipathic antimicrobial peptide, melittin, expressed within recombinant plasmid vectors, on infection with urogenital pathogens Chlamydia trachomatis and Mycoplasma hominis in HeLa cell culture. Recombinant plasmid constructs with the melittin gene under the control of the tetracycline-responsive promoter of human cytomegalovirus were obtained. We showed inhibition of C. trachomatis and M. hominis infection after the introduction of recombinant plasmid vectors expressing the melittin gene into the infected cell culture.
AbstractList As the number of pathogenic microbial strains resistant to different antibiotics increases, amphipathic peptides with antimicrobial activity are promising agents for the therapy of infectious diseases. This work deals with the effect of an amphipathic antimicrobial peptide, melittin, expressed within recombinant plasmid vectors, on infection with urogenital pathogens Chlamydia trachomatis and Mycoplasma hominis in HeLa cell culture. Recombinant plasmid constructs with the melittin gene under the control of the tetracycline-responsive promoter of human cytomegalovirus were obtained. We showed inhibition of C. trachomatis and M. hominis infection after the introduction of recombinant plasmid vectors expressing the melittin gene into the infected cell culture.
Author LAZAREV, V. N
AKOPIAN, T. A
MISYURINA, O. Yu
GULARYAN, S. K
PARFENOVA, T. M
GOVORUN, V. M
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Snippet As the number of pathogenic microbial strains resistant to different antibiotics increases, amphipathic peptides with antimicrobial activity are promising...
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SubjectTerms Chlamydia trachomatis - drug effects
Chlamydia trachomatis - physiology
Cytomegalovirus - genetics
Gene Expression Regulation - drug effects
Genetic Vectors - genetics
HeLa Cells
Humans
Melitten - biosynthesis
Melitten - genetics
Melitten - pharmacology
Membrane Potentials
Mycoplasma hominis - drug effects
Mycoplasma hominis - physiology
Promoter Regions, Genetic - genetics
Tetracycline - pharmacology
Transfection
Title Induced expression of melittin, an antimicrobial peptide, inhibits infection by Chlamydia trachomatis and Mycoplasma hominis in a HeLa cell line
URI https://www.ncbi.nlm.nih.gov/pubmed/11850166
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