Determination of ploidy level in “Mitchell” Petunias

• Published in: Plant science (Limerick) Vol. 65; no. 1; pp. 119 - 124
• Main Authors: Kamo, Kathryn K., Griesbach, Robert J.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1989; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Cytogenetics; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetics and breeding of economic plants; Genetics of eukaryotes. Biological and molecular evolution; microfluorimetry; ploidy determinations; Pteridophyta, spermatophyta; Vegetals and fungi; “Mitchell” Petunias; microfluorimetry; “Mitchell” Petunias; ploidy determinations; Ploidy; Tissue culture; Chromosomic number; Fluorescence spectrometry; Haploidy; Regeneration; Diploidy; Petunia; Dicotyledones; Angiospermae; Flower crop; Spermatophyta; Solanaceae; Chimerism; Chloroplast; Cultivar

Mitchell Petunia plants were analyzed for their level of ploidy by: (1) counting the number of chromosomes in root tips; (2) counting the number of chloroplasts per guard cell pair; and (3) using fluorescence microscopy to measure the amount of DNA stained with Hoechst 33258. The majority of plants...