Identification of Borrelia burgdorferi sensu lato Tick Isolates from Slovakia by PCR Typing with 16S rRNA Primers

• Published in: Zentralblatt für Bakteriologie Vol. 286; no. 3; pp. 355 - 361
• Main Authors: Mateička, F., Kozáková, Darina, Rosa, Patricia A., Kmety, E.
• Format: Journal Article
• Language: English
• Published: Jena Urban & Fischer 01.10.1997
• Subjects: Animals; Antibodies, Monoclonal; Antigens, Bacterial - analysis; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Blotting, Western; Borrelia burgdorferi Group - genetics; Borrelia burgdorferi Group - immunology; Borrelia burgdorferi Group - isolation & purification; DNA, Bacterial - analysis; Fundamental and applied biological sciences. Psychology; Mice; Microbiology; Plasmids; Polymerase Chain Reaction; RNA, Ribosomal, 16S - genetics; Slovakia; Ticks; Europe; Slovakia; Spirochaetaceae; Ribosomal DNA; Identification; Apodemus flavicollis; Epidemiology; Acari; Borrelia garinii; 16S-RNA; Bacteria; Vector; Ixodidae; Parasitiformes; Borrelia burgdorferi; Spirochaetales; Rodentia; Genotype; Polymerase chain reaction; Arachnida; Vertebrata; Mammalia; Ixodida; Arthropoda; Intermediate host; Ixodes ricinus; Invertebrata

The first four strains of Borrelia burgdorferi isolated in Slovakia from ticks and mice were studied using monoclonal antibodies, the polymerase chain reaction (PCR) with 16S rRNA specific primers and plasmid profiles. Two tick isolates were typed as Borrelia garinii, one strain isolated from Apodem...