Determination of anti-neutrophil cytoplasm antibodies (ANCA) specificity by immunofluorescence on chronic myelocytic leukemia cells

• Published in: Journal of immunological methods Vol. 147; no. 1; p. 101
• Main Authors: Chevailler, A, Noel, L H, Renier, G, Gardembas-Pain, M, Subra, J F, Nusbaum, P, Hurez, D, Lesavre, P
• Format: Journal Article
• Language: English
• Published: Netherlands 14.02.1992
• Subjects: Antibodies, Antineutrophil Cytoplasmic; Antibodies, Antinuclear - immunology; Antibody Specificity; Autoantibodies - analysis; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Granulomatosis with Polyangiitis - immunology; Humans; Immunoglobulin G - analysis; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - immunology; Neutrophils - immunology; Peroxidase - deficiency; Peroxidase - immunology

ANCA positive sera, detected by the standard immunofluorescence method, derived from 37 patients with vasculitis were studied using formalin-acetone fixed chronic myelocytic leukemia cells (CML). All 37 sera were positive on CML cell smears. Furthermore formalin-actone fixation selectively impaired...