Recognition of nuclear export signals by CRM1 carrying the oncogenic E571K mutation

• Published in: Molecular biology of the cell Vol. 31; no. 17; pp. 1879 - 1891
• Main Authors: Baumhardt, Jordan M, Walker, Janek S, Lee, Yoonji, Shakya, Binita, Brautigam, Chad A, Lapalombella, Rosa, Grishin, Nick, Chook, Yuh Min
• Format: Journal Article
• Language: English
• Published: United States American Society for Cell Biology 01.08.2020; The American Society for Cell Biology
• Series: A Highlights from
• Subjects: Active Transport, Cell Nucleus; Amino Acid Sequence - genetics; BASIC BIOLOGICAL SCIENCES; Cell Nucleus - metabolism; Crystallography, X-Ray - methods; Exportin 1 Protein; HEK293 Cells; Humans; Karyopherins - genetics; Karyopherins - metabolism; MAP Kinase Kinase 1 - metabolism; Models, Molecular; Mutation - genetics; Nuclear Export Signals - genetics; Nucleocytoplasmic Transport Proteins - metabolism; Protein Binding - genetics; ran GTP-Binding Protein - genetics; ran GTP-Binding Protein - metabolism; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Ribosomal Proteins - metabolism

The E571K mutation of CRM1 is highly prevalent in some cancers, but its mechanism of tumorigenesis is unclear. Glu571 of CRM1 is located in its nuclear export signal (NES)-binding groove, suggesting that binding of select NESs may be altered. We generated HEK 293 cells with either monoallelic CRM1WT...