Self-organized optimal packing of kinesin-5-driven microtubule asters scales with cell size

Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form intracellular patterns, but the mechanical principles behind this are not clear. Here, we report that oocytes of the marine ascidian Phallusia ma...

Full description

Saved in:
Bibliographic Details
Published inJournal of cell science Vol. 134; no. 10
Main Authors Khetan, Neha, Pruliere, Gérard, Hebras, Celine, Chenevert, Janet, Athale, Chaitanya A.
Format Journal Article
LanguageEnglish
Published Company of Biologists 15.05.2021
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form intracellular patterns, but the mechanical principles behind this are not clear. Here, we report that oocytes of the marine ascidian Phallusia mammillata treated with the drug BI-D1870 spontaneously form cytoplasmic MT asters, or cytasters. These asters form steady state segregation patterns in a shell just under the membrane. Cytaster centers tessellate the oocyte cytoplasm, that is divide it into polygonal structures, dominated by hexagons, in a kinesin-5-dependent manner, while inter-aster MTs form ‘mini-spindles’. A computational model of multiple asters interacting with kinesin-5 can reproduce both tessellation patterns and mini-spindles in a manner specific to the number of MTs per aster, MT lengths and kinesin-5 density. Simulations predict that the hexagonal tessellation patterns scale with increasing cell size, when the packing fraction of asters in cells is ∼1.6. This self-organized in vivo tessellation by cytasters is comparable to the ‘circle packing problem’, suggesting that there is an intrinsic mechanical pattern-forming module that is potentially relevant to understanding the role of collective mechanics of cytoskeletal elements in embryogenesis. This article has an associated First Person interview with the first author of the paper.
AbstractList ABSTRACT Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form intracellular patterns, but the mechanical principles behind this are not clear. Here, we report that oocytes of the marine ascidian Phallusia mammillata treated with the drug BI-D1870 spontaneously form cytoplasmic MT asters, or cytasters. These asters form steady state segregation patterns in a shell just under the membrane. Cytaster centers tessellate the oocyte cytoplasm, that is divide it into polygonal structures, dominated by hexagons, in a kinesin-5-dependent manner, while inter-aster MTs form ‘mini-spindles’. A computational model of multiple asters interacting with kinesin-5 can reproduce both tessellation patterns and mini-spindles in a manner specific to the number of MTs per aster, MT lengths and kinesin-5 density. Simulations predict that the hexagonal tessellation patterns scale with increasing cell size, when the packing fraction of asters in cells is ∼1.6. This self-organized in vivo tessellation by cytasters is comparable to the ‘circle packing problem’, suggesting that there is an intrinsic mechanical pattern-forming module that is potentially relevant to understanding the role of collective mechanics of cytoskeletal elements in embryogenesis.
Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form intracellular patterns, but the mechanical principles behind this are not clear. Here, we report that oocytes of the marine ascidian Phallusia mammillata treated with the drug BI-D1870 spontaneously form cytoplasmic MT asters, or cytasters. These asters form steady state segregation patterns in a shell just under the membrane. Cytaster centers tessellate the oocyte cytoplasm, that is divide it into polygonal structures, dominated by hexagons, in a kinesin-5-dependent manner, while inter-aster MTs form ‘mini-spindles’. A computational model of multiple asters interacting with kinesin-5 can reproduce both tessellation patterns and mini-spindles in a manner specific to the number of MTs per aster, MT lengths and kinesin-5 density. Simulations predict that the hexagonal tessellation patterns scale with increasing cell size, when the packing fraction of asters in cells is ∼1.6. This self-organized in vivo tessellation by cytasters is comparable to the ‘circle packing problem’, suggesting that there is an intrinsic mechanical pattern-forming module that is potentially relevant to understanding the role of collective mechanics of cytoskeletal elements in embryogenesis. This article has an associated First Person interview with the first author of the paper.
Author Athale, Chaitanya A.
Khetan, Neha
Pruliere, Gérard
Chenevert, Janet
Hebras, Celine
Author_xml – sequence: 1
  givenname: Neha
  orcidid: 0000-0001-9972-1921
  surname: Khetan
  fullname: Khetan, Neha
– sequence: 2
  givenname: Gérard
  surname: Pruliere
  fullname: Pruliere, Gérard
– sequence: 3
  givenname: Celine
  surname: Hebras
  fullname: Hebras, Celine
– sequence: 4
  givenname: Janet
  orcidid: 0000-0002-8231-4008
  surname: Chenevert
  fullname: Chenevert, Janet
– sequence: 5
  givenname: Chaitanya A.
  orcidid: 0000-0002-9506-2153
  surname: Athale
  fullname: Athale, Chaitanya A.
BackLink https://hal.science/hal-03435922$$DView record in HAL
BookMark eNo9kM9LwzAYhoMouE0v_gU5qpCZn017HEOdMPCgnjyEtE22zKypSTvRv96OiqcXPh4e3u-dgtMmNAaAK4LnhHJ6t6vSnAopODsBE8KlRAVh8hRMMKYEFYKxczBNaYcxlrSQE_D-YrxFIW50435MDUPbub32sNXVh2s2MFg4pEmuQQLV0R1MA_euiqHry94bqFNnYoKp0t4k-OW6LayM9zANtgtwZrVP5vIvZ-Dt4f51uULr58en5WKNKsZ4hyzOS6ptLXOGM1PJXFiuKSNZVgqrLaOU11RrriWtrSgLygkVdW6ItrbMc8Nm4Gb0brVXbRz6x28VtFOrxVodb5hxJgpKD2Rgr0e2jeGzN6lTe5eOjXVjQp8UFSyThWRFPqC3Izp8m1I09t9NsDqurYa11bg2-wXV3XSg
CitedBy_id crossref_primary_10_7554_eLife_63880
crossref_primary_10_1242_dev_200851
crossref_primary_10_26508_lsa_202302427
crossref_primary_10_1103_PhysRevLett_129_268003
crossref_primary_10_1242_jcs_258883
crossref_primary_10_1103_PhysRevE_108_024404
Cites_doi 10.1016/j.jtbi.2014.06.001
10.1039/c1ib00059d
10.1088/1478-3975/11/1/016008
10.1083/jcb.201006076
10.1083/jcb.201204019
10.1103/PhysRevLett.70.1347
10.1016/j.cub.2007.11.049
10.1016/j.cell.2007.06.025
10.1126/science.1198343
10.1242/jcs.102.3.557
10.1006/dbio.2000.9931
10.1126/science.1084146
10.1038/100021a0
10.1242/jcs.73.1.261
10.1371/journal.pcbi.1005102
10.1016/S0092-8674(00)80992-7
10.1103/PhysRevLett.86.3192
10.1042/BJ20110289
10.1242/jcs.61.1.175
10.1038/nmeth.2089
10.1038/9025
10.1083/jcb.201202135
10.1088/1367-2630/9/11/427
10.1002/cm.20283
10.1073/pnas.0812493106
10.1083/jcb.201510064
10.1016/bs.mcb.2015.01.015
10.1016/S0960-9822(07)00370-3
10.1126/science.1115964
10.1016/j.cell.2012.01.007
10.4161/cib.2.4.8240
10.1073/pnas.0710311105
10.1103/PhysRevLett.105.128103
10.1126/science.1161820
10.1016/bs.mcb.2015.03.006
10.1016/j.devcel.2005.03.007
10.1091/mbc.e16-06-0395
10.1016/j.devcel.2010.11.011
10.1006/dbio.2000.9726
10.1083/jcb.122.1.113
10.1038/35054572
10.1038/38532
10.1088/1367-2630/16/1/013018
10.1083/jcb.200202051
10.1016/j.celrep.2014.07.015
10.1111/boc.201300054
10.1038/s41592-019-0686-2
10.1083/jcb.94.2.455
10.1073/pnas.1013275108
10.1016/j.cub.2003.09.006
10.4161/cc.8.8.8150
10.1016/j.celrep.2020.108213
10.1038/emboj.2008.242
10.4161/cc.21753
10.1073/pnas.94.12.6228
10.1038/ncb1332
10.1083/jcb.118.5.1097
10.1201/9781315119601
10.1038/emm.2016.61
10.3390/cells9051150
10.1039/D0SM01086C
10.1073/pnas.95.8.4293
10.1111/j.1600-0854.2007.00698.x
10.1091/mbc.e10-07-0627
10.1016/B978-0-12-394308-8.00006-6
10.1016/j.ydbio.2010.10.017
10.1016/j.devcel.2005.03.014
10.3390/cells9020505
10.1126/science.aaz5635
10.1073/pnas.252407099
10.1042/BJ20070797
10.1016/j.cub.2009.09.025
10.1007/s00249-007-0134-6
10.1083/jcb.120.4.923
10.1038/s41598-020-57484-y
10.1126/science.aav7793
10.1042/BJ20061088
10.1016/j.devcel.2018.09.011
ContentType Journal Article
Copyright Distributed under a Creative Commons Attribution 4.0 International License
Copyright_xml – notice: Distributed under a Creative Commons Attribution 4.0 International License
DBID AAYXX
CITATION
7X8
1XC
VOOES
DOI 10.1242/jcs.257543
DatabaseName CrossRef
MEDLINE - Academic
Hyper Article en Ligne (HAL)
Hyper Article en Ligne (HAL) (Open Access)
DatabaseTitle CrossRef
MEDLINE - Academic
DatabaseTitleList
CrossRef
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1477-9137
ExternalDocumentID oai_HAL_hal_03435922v1
10_1242_jcs_257543
GroupedDBID ---
-DZ
-~X
0R~
18M
2WC
34G
39C
4.4
53G
5GY
5RE
5VS
85S
AAYXX
ABDNZ
ABJNI
ABPPZ
ACGFO
ACGFS
ACIWK
ACNCT
ACPRK
ADBBV
ADCOW
AEILP
AENEX
AFFNX
AFRAH
AGGIJ
ALMA_UNASSIGNED_HOLDINGS
BAWUL
BTFSW
CITATION
CS3
DIK
DU5
E3Z
EBS
F5P
F9R
GX1
HZ~
IH2
INIJC
KQ8
O9-
OK1
P2P
R.V
RCB
RHF
RHI
RNS
SJN
TN5
TR2
UPT
W2D
W8F
WH7
WOQ
YQT
~02
~KM
7X8
1XC
VOOES
ID FETCH-LOGICAL-c334t-f08b2afd78306ec785f4a23166b5faf3224d2aa4a72df5b924125d8e1affb88e3
ISSN 0021-9533
IngestDate Thu Oct 31 07:23:06 EDT 2024
Thu Oct 24 23:48:54 EDT 2024
Fri Dec 06 08:25:37 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 10
Keywords kinesin-5
scaling
aster
self-organization
Microtubule
tessellation
Language English
License http://www.biologists.com/user-licence-1-1
Distributed under a Creative Commons Attribution 4.0 International License: http://creativecommons.org/licenses/by/4.0
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c334t-f08b2afd78306ec785f4a23166b5faf3224d2aa4a72df5b924125d8e1affb88e3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0001-9972-1921
0000-0002-9506-2153
0000-0002-8231-4008
OpenAccessLink https://hal.science/hal-03435922
PQID 2536797398
PQPubID 23479
ParticipantIDs hal_primary_oai_HAL_hal_03435922v1
proquest_miscellaneous_2536797398
crossref_primary_10_1242_jcs_257543
PublicationCentury 2000
PublicationDate 20210515
PublicationDateYYYYMMDD 2021-05-15
PublicationDate_xml – month: 05
  year: 2021
  text: 20210515
  day: 15
PublicationDecade 2020
PublicationTitle Journal of cell science
PublicationYear 2021
Publisher Company of Biologists
Publisher_xml – name: Company of Biologists
References Boleti (2024061311310930300_JCS257543C6) 1996; 84
Kimura (2024061311310930300_JCS257543C33) 2005; 8
Grill (2024061311310930300_JCS257543C21) 2005; 8
Yang (2024061311310930300_JCS257543C79) 2016; 6
Burakov (2024061311310930300_JCS257543C7) 2008; 9
Soeda (2024061311310930300_JCS257543C64) 2018; 47
Walczak (2024061311310930300_JCS257543C76) 1998; 8
Corson (2024061311310930300_JCS257543C13) 2009; 106
Malikov (2024061311310930300_JCS257543C45) 2005; 7
Romeo (2024061311310930300_JCS257543C57) 2012; 441
Lagrange (2024061311310930300_JCS257543C83) 1773
Hornick (2024061311310930300_JCS257543C25) 2008; 65
Athale (2024061311310930300_JCS257543C1) 2008; 322
Laan (2024061311310930300_JCS257543C39) 2012; 11
Caudron (2024061311310930300_JCS257543C10) 2005; 309
Courtois (2024061311310930300_JCS257543C14) 2012; 198
Tanenbaum (2024061311310930300_JCS257543C68) 2010; 19
Cantillana (2024061311310930300_JCS257543C8) 2000; 228
Holy (2024061311310930300_JCS257543C24) 1997; 94
Korneev (2024061311310930300_JCS257543C35) 2007; 36
Liu (2024061311310930300_JCS257543C42) 2020; 10
McDougall (2024061311310930300_JCS257543C48) 2015; 129
Gittes (2024061311310930300_JCS257543C19) 1993; 120
Ferenz (2024061311310930300_JCS257543C18) 2009; 19
Loughlin (2024061311310930300_JCS257543C43) 2010; 191
Ma (2024061311310930300_JCS257543C44) 2014; 16
Mitchison (2024061311310930300_JCS257543C51) 2019; 366
Tanenbaum (2024061311310930300_JCS257543C69) 2008; 27
Cheng (2024061311310930300_JCS257543C11) 2019; 366
Waitzman (2024061311310930300_JCS257543C75) 2014; 106
Gauss (2024061311310930300_JCS257543C82) 1831; 20
Laan (2024061311310930300_JCS257543C37) 2008; 105
Schneider (2024061311310930300_JCS257543C61) 2012; 9
Virtanen (2024061311310930300_JCS257543C27) 2020; 17
Letort (2024061311310930300_JCS257543C41) 2016; 27
Kuriyama (2024061311310930300_JCS257543C36) 1983; 61
Zhu (2024061311310930300_JCS257543C80) 2010; 21
Verde (2024061311310930300_JCS257543C74) 1992; 118
Surrey (2024061311310930300_JCS257543C66) 1998; 95
Faivre-Moskalenko (2024061311310930300_JCS257543C16) 2002; 99
Koke (2024061311310930300_JCS257543C34) 2014; 359
Grill (2024061311310930300_JCS257543C22) 2001; 409
Kallenbach (2024061311310930300_JCS257543C28) 1985; 73
Sapkota (2024061311310930300_JCS257543C59) 2007; 401
Meaders (2024061311310930300_JCS257543C49) 2020; 9
Khetan (2024061311310930300_JCS257543C31) 2020; 16
van Heesbeen (2024061311310930300_JCS257543C73) 2014; 8
Nédélec (2024061311310930300_JCS257543C55) 2001; 86
Sachs (2024061311310930300_JCS257543C58) 1892; 75
Stephano (2024061311310930300_JCS257543C65) 2000; 222
Baker (2024061311310930300_JCS257543C4) 1993; 122
Schuh (2024061311310930300_JCS257543C62) 2007; 130
Park (2024061311310930300_JCS257543C56) 2016; 48
Athale (2024061311310930300_JCS257543C2) 2014; 11
Telley (2024061311310930300_JCS257543C71) 2012; 197
McDougall (2024061311310930300_JCS257543C47) 2012; 297
Fejes Tóth (2024061311310930300_JCS257543C81) 2017
Wühr (2024061311310930300_JCS257543C78) 2009; 8
Dogterom (2024061311310930300_JCS257543C15) 1993; 70
Schatten (2024061311310930300_JCS257543C60) 1982; 94
Nedelec (2024061311310930300_JCS257543C53) 2007; 9
Harris (2024061311310930300_JCS257543C23) 1992; 102
Colombo (2024061311310930300_JCS257543C12) 2003; 300
Laan (2024061311310930300_JCS257543C38) 2012; 148
Carazo-Salas (2024061311310930300_JCS257543C9) 2003; 13
Khetan (2024061311310930300_JCS257543C30) 2016; 12
Nédélec (2024061311310930300_JCS257543C52) 2002; 158
Nédélec (2024061311310930300_JCS257543C54) 1997; 389
Kimura (2024061311310930300_JCS257543C32) 2010; 108
Tanimoto (2024061311310930300_JCS257543C70) 2016; 212
Meaders (2024061311310930300_JCS257543C50) 2020; 33
Mavrakis (2024061311310930300_JCS257543C46) 2009; 2
Farhadifar (2024061311310930300_JCS257543C17) 2007; 17
Leduc (2024061311310930300_JCS257543C40) 2010; 105
Bain (2024061311310930300_JCS257543C3) 2007; 408
Sharp (2024061311310930300_JCS257543C63) 1999; 1
Tamulonis (2024061311310930300_JCS257543C67) 2011; 351
Baumann (2024061311310930300_JCS257543C5) 2015; 128
Grill (2024061311310930300_JCS257543C20) 2010; 330
Jessus (2024061311310930300_JCS257543C26) 2020; 9
Kanesaki (2024061311310930300_JCS257543C29) 2011; 3
Thompson (2024061311310930300_JCS257543C72) 1917; 100
References_xml – volume: 359
  start-page: 92
  year: 2014
  ident: 2024061311310930300_JCS257543C34
  article-title: A computational model of nuclear self-organisation in syncytial embryos
  publication-title: J. Theor. Biol.
  doi: 10.1016/j.jtbi.2014.06.001
  contributor:
    fullname: Koke
– volume: 3
  start-page: 1112
  year: 2011
  ident: 2024061311310930300_JCS257543C29
  article-title: Dynamic ordering of nuclei in syncytial embryos: a quantitative analysis of the role of cytoskeletal networks
  publication-title: Integr. Biol.
  doi: 10.1039/c1ib00059d
  contributor:
    fullname: Kanesaki
– volume: 11
  start-page: 16008
  year: 2014
  ident: 2024061311310930300_JCS257543C2
  article-title: Collective behavior of minus-ended motors in mitotic microtubule asters gliding toward DNA
  publication-title: Phys. Biol.
  doi: 10.1088/1478-3975/11/1/016008
  contributor:
    fullname: Athale
– volume: 191
  start-page: 1239
  year: 2010
  ident: 2024061311310930300_JCS257543C43
  article-title: A computational model predicts Xenopus meiotic spindle organization
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201006076
  contributor:
    fullname: Loughlin
– volume: 197
  start-page: 887
  year: 2012
  ident: 2024061311310930300_JCS257543C71
  article-title: Aster migration determines the length scale of nuclear separation in the Drosophila syncytial embryo
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201204019
  contributor:
    fullname: Telley
– volume: 70
  start-page: 1347
  year: 1993
  ident: 2024061311310930300_JCS257543C15
  article-title: Physical aspects of the growth and regulation of microtubule structures
  publication-title: Phys. Rev. Lett.
  doi: 10.1103/PhysRevLett.70.1347
  contributor:
    fullname: Dogterom
– volume: 17
  start-page: 2095
  year: 2007
  ident: 2024061311310930300_JCS257543C17
  article-title: The influence of cell mechanics, cell-cell interactions, and proliferation on epithelial packing
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2007.11.049
  contributor:
    fullname: Farhadifar
– volume: 130
  start-page: 484
  year: 2007
  ident: 2024061311310930300_JCS257543C62
  article-title: Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes
  publication-title: Cell
  doi: 10.1016/j.cell.2007.06.025
  contributor:
    fullname: Schuh
– volume: 330
  start-page: 597
  year: 2010
  ident: 2024061311310930300_JCS257543C20
  article-title: Cell biology. forced to be unequal
  publication-title: Science
  doi: 10.1126/science.1198343
  contributor:
    fullname: Grill
– volume: 102
  start-page: 557
  year: 1992
  ident: 2024061311310930300_JCS257543C23
  article-title: Conditions for assembly of tubulin-based structures in unfertilized sea urchin eggs. Spirals, monasters and cytasters
  publication-title: J. Cell. Sci.
  doi: 10.1242/jcs.102.3.557
  contributor:
    fullname: Harris
– volume: 228
  start-page: 136
  year: 2000
  ident: 2024061311310930300_JCS257543C8
  article-title: The complex dynamic network of microtubule and microfilament cytasters of the leech zygote
  publication-title: Dev. Biol.
  doi: 10.1006/dbio.2000.9931
  contributor:
    fullname: Cantillana
– volume: 300
  start-page: 1957
  year: 2003
  ident: 2024061311310930300_JCS257543C12
  article-title: Translation of polarity cues into asymmetric spindle positioning in Caenorhabditis elegans embryos
  publication-title: Science
  doi: 10.1126/science.1084146
  contributor:
    fullname: Colombo
– volume: 100
  start-page: 21
  year: 1917
  ident: 2024061311310930300_JCS257543C72
  article-title: On growth and form
  publication-title: Nature
  doi: 10.1038/100021a0
  contributor:
    fullname: Thompson
– volume: 73
  start-page: 261
  year: 1985
  ident: 2024061311310930300_JCS257543C28
  article-title: Ultrastructural analysis of the initiation and development of cytasters in sea-urchin eggs
  publication-title: J. Cell. Sci.
  doi: 10.1242/jcs.73.1.261
  contributor:
    fullname: Kallenbach
– volume: 12
  start-page: e1005102
  year: 2016
  ident: 2024061311310930300_JCS257543C30
  article-title: A motor-gradient and clustering model of the centripetal motility of MTOCs in meiosis I of mouse oocytes
  publication-title: PLoS Comput. Biol.
  doi: 10.1371/journal.pcbi.1005102
  contributor:
    fullname: Khetan
– volume: 84
  start-page: 49
  year: 1996
  ident: 2024061311310930300_JCS257543C6
  article-title: Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis
  publication-title: Cell
  doi: 10.1016/S0092-8674(00)80992-7
  contributor:
    fullname: Boleti
– volume: 86
  start-page: 3192
  year: 2001
  ident: 2024061311310930300_JCS257543C55
  article-title: Dynamic concentration of motors in microtubule arrays
  publication-title: Phys. Rev. Lett.
  doi: 10.1103/PhysRevLett.86.3192
  contributor:
    fullname: Nédélec
– volume: 441
  start-page: 553
  year: 2012
  ident: 2024061311310930300_JCS257543C57
  article-title: Regulation and function of the RSK family of protein kinases
  publication-title: Biochem. J.
  doi: 10.1042/BJ20110289
  contributor:
    fullname: Romeo
– volume: 61
  start-page: 175
  year: 1983
  ident: 2024061311310930300_JCS257543C36
  article-title: Cytasters induced within unfertilized sea-urchin eggs
  publication-title: J. Cell. Sci.
  doi: 10.1242/jcs.61.1.175
  contributor:
    fullname: Kuriyama
– volume: 9
  start-page: 671
  year: 2012
  ident: 2024061311310930300_JCS257543C61
  article-title: NIH Image to ImageJ: 25 years of image analysis
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.2089
  contributor:
    fullname: Schneider
– volume: 1
  start-page: 51
  year: 1999
  ident: 2024061311310930300_JCS257543C63
  article-title: Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos
  publication-title: Nat. Cell Biol.
  doi: 10.1038/9025
  contributor:
    fullname: Sharp
– volume: 198
  start-page: 357
  year: 2012
  ident: 2024061311310930300_JCS257543C14
  article-title: The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201202135
  contributor:
    fullname: Courtois
– volume: 9
  start-page: 427
  year: 2007
  ident: 2024061311310930300_JCS257543C53
  article-title: Collective Langevin dynamics of flexible cytoskeletal fibers
  publication-title: New J. Phys.
  doi: 10.1088/1367-2630/9/11/427
  contributor:
    fullname: Nedelec
– start-page: 693
  volume-title: Nouv. Mém. Acad. Roy. Belles Letters Berlin, 265–312: Œuvres complète (1869)
  year: 1773
  ident: 2024061311310930300_JCS257543C83
  article-title: Recherches d’arithmétique
  contributor:
    fullname: Lagrange
– volume: 65
  start-page: 595
  year: 2008
  ident: 2024061311310930300_JCS257543C25
  article-title: Live-cell analysis of mitotic spindle formation in taxol-treated cells
  publication-title: Cell Motil. Cytoskeleton
  doi: 10.1002/cm.20283
  contributor:
    fullname: Hornick
– volume: 106
  start-page: 8453
  year: 2009
  ident: 2024061311310930300_JCS257543C13
  article-title: Turning a plant tissue into a living cell froth through isotropic growth
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0812493106
  contributor:
    fullname: Corson
– volume: 212
  start-page: 777
  year: 2016
  ident: 2024061311310930300_JCS257543C70
  article-title: Shape–motion relationships of centering microtubule asters
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.201510064
  contributor:
    fullname: Tanimoto
– volume: 128
  start-page: 39
  year: 2015
  ident: 2024061311310930300_JCS257543C5
  article-title: Self-organization of motors and microtubules in lipid-monolayered droplets
  publication-title: Methods Cell Biol.
  doi: 10.1016/bs.mcb.2015.01.015
  contributor:
    fullname: Baumann
– volume: 8
  start-page: 903
  year: 1998
  ident: 2024061311310930300_JCS257543C76
  article-title: A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity
  publication-title: Curr. Biol.
  doi: 10.1016/S0960-9822(07)00370-3
  contributor:
    fullname: Walczak
– volume: 309
  start-page: 1373
  year: 2005
  ident: 2024061311310930300_JCS257543C10
  article-title: Spatial coordination of spindle assembly by chromosome-mediated signaling gradients
  publication-title: Science
  doi: 10.1126/science.1115964
  contributor:
    fullname: Caudron
– volume: 148
  start-page: 502
  year: 2012
  ident: 2024061311310930300_JCS257543C38
  article-title: Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters
  publication-title: Cell
  doi: 10.1016/j.cell.2012.01.007
  contributor:
    fullname: Laan
– volume: 2
  start-page: 313
  year: 2009
  ident: 2024061311310930300_JCS257543C46
  article-title: Cells within a cell: Insights into cellular architecture and polarization from the organization of the early fly embryo
  publication-title: Commun. Integr. Biol.
  doi: 10.4161/cib.2.4.8240
  contributor:
    fullname: Mavrakis
– volume: 105
  start-page: 8920
  year: 2008
  ident: 2024061311310930300_JCS257543C37
  article-title: Force-generation and dynamic instability of microtubule bundles
  publication-title: Proc. Natl. Acad. Sci. U.S.A.
  doi: 10.1073/pnas.0710311105
  contributor:
    fullname: Laan
– volume: 105
  start-page: 1
  year: 2010
  ident: 2024061311310930300_JCS257543C40
  article-title: Collective behavior of antagonistically acting kinesin-1 motors
  publication-title: Phys. Rev. Lett.
  doi: 10.1103/PhysRevLett.105.128103
  contributor:
    fullname: Leduc
– volume: 322
  start-page: 1243
  year: 2008
  ident: 2024061311310930300_JCS257543C1
  article-title: Regulation of microtubule dynamics by reaction cascades around chromosomes
  publication-title: Science
  doi: 10.1126/science.1161820
  contributor:
    fullname: Athale
– volume: 129
  start-page: 317
  year: 2015
  ident: 2024061311310930300_JCS257543C48
  article-title: Centrosomes and spindles in ascidian embryos and eggs
  publication-title: Methods Cell Biol.
  doi: 10.1016/bs.mcb.2015.03.006
  contributor:
    fullname: McDougall
– volume: 8
  start-page: 765
  year: 2005
  ident: 2024061311310930300_JCS257543C33
  article-title: Computer simulations and image processing reveal length-dependent pulling force as the primary mechanism for C. elegans male pronuclear migration
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2005.03.007
  contributor:
    fullname: Kimura
– volume: 27
  start-page: 2833
  year: 2016
  ident: 2024061311310930300_JCS257543C41
  article-title: Centrosome centering and decentering by microtubule network rearrangement
  publication-title: Mol. Biol. Cell
  doi: 10.1091/mbc.e16-06-0395
  contributor:
    fullname: Letort
– volume: 19
  start-page: 797
  year: 2010
  ident: 2024061311310930300_JCS257543C68
  article-title: Mechanisms of centrosome separation and bipolar spindle assembly
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2010.11.011
  contributor:
    fullname: Tanenbaum
– volume: 222
  start-page: 420
  year: 2000
  ident: 2024061311310930300_JCS257543C65
  article-title: MAP kinase, a universal suppressor of sperm centrosomes during meiosis?
  publication-title: Dev. Biol.
  doi: 10.1006/dbio.2000.9726
  contributor:
    fullname: Stephano
– volume: 122
  start-page: 113
  year: 1993
  ident: 2024061311310930300_JCS257543C4
  article-title: Dynamic changes in microtubule configuration correlate with nuclear migration in the preblastoderm Drosophila embryo
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.122.1.113
  contributor:
    fullname: Baker
– volume: 409
  start-page: 630
  year: 2001
  ident: 2024061311310930300_JCS257543C22
  article-title: Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo
  publication-title: Nature
  doi: 10.1038/35054572
  contributor:
    fullname: Grill
– volume: 389
  start-page: 305
  year: 1997
  ident: 2024061311310930300_JCS257543C54
  article-title: Self-organization of microtubules and motors
  publication-title: Nature
  doi: 10.1038/38532
  contributor:
    fullname: Nédélec
– volume: 16
  start-page: 13018
  year: 2014
  ident: 2024061311310930300_JCS257543C44
  article-title: General theory for the mechanics of confined microtubule asters
  publication-title: New J. Phys.
  doi: 10.1088/1367-2630/16/1/013018
  contributor:
    fullname: Ma
– volume: 158
  start-page: 1005
  year: 2002
  ident: 2024061311310930300_JCS257543C52
  article-title: Computer simulations reveal motor properties generating stable antiparallel microtubule interactions
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.200202051
  contributor:
    fullname: Nédélec
– volume: 8
  start-page: 948
  year: 2014
  ident: 2024061311310930300_JCS257543C73
  article-title: Balanced activity of three mitotic motors is required for bipolar spindle assembly and chromosome segregation
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2014.07.015
  contributor:
    fullname: van Heesbeen
– volume: 106
  start-page: 1
  year: 2014
  ident: 2024061311310930300_JCS257543C75
  article-title: Mechanism and regulation of kinesin-5, an essential motor for the mitotic spindle
  publication-title: Biol. Cell
  doi: 10.1111/boc.201300054
  contributor:
    fullname: Waitzman
– volume: 17
  start-page: 261
  year: 2020
  ident: 2024061311310930300_JCS257543C27
  article-title: SciPy 1.0: fundamental algorithms for scientific computing in Python
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0686-2
  contributor:
    fullname: Virtanen
– volume: 75
  start-page: 57
  year: 1892
  ident: 2024061311310930300_JCS257543C58
  article-title: Physiologische notizen, II. Beiträge zur zelltheorie. energiden und zellen
  publication-title: Flora
  contributor:
    fullname: Sachs
– volume: 94
  start-page: 455
  year: 1982
  ident: 2024061311310930300_JCS257543C60
  article-title: Taxol inhibits the nuclear movements during fertilization and induces asters in unfertilized sea urchin eggs
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.94.2.455
  contributor:
    fullname: Schatten
– volume: 6
  start-page: 1
  year: 2016
  ident: 2024061311310930300_JCS257543C79
  article-title: Kinesin-5 contributes to spindle-length scaling in the evolution of cancer toward metastasis
  publication-title: Sci. Rep.
  contributor:
    fullname: Yang
– volume: 108
  start-page: 137
  year: 2010
  ident: 2024061311310930300_JCS257543C32
  article-title: Intracellular organelles mediate cytoplasmic pulling force for centrosome centration in the Caenorhabditis elegans early embryo
  publication-title: Proc. Nat. Acad. Sci. USA
  doi: 10.1073/pnas.1013275108
  contributor:
    fullname: Kimura
– volume: 13
  start-page: 1728
  year: 2003
  ident: 2024061311310930300_JCS257543C9
  article-title: Long-range communication between chromatin and microtubules in Xenopus egg extracts
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2003.09.006
  contributor:
    fullname: Carazo-Salas
– volume: 8
  start-page: 1115
  year: 2009
  ident: 2024061311310930300_JCS257543C78
  article-title: How does a millimeter-sized cell find its center?
  publication-title: Cell Cycle
  doi: 10.4161/cc.8.8.8150
  contributor:
    fullname: Wühr
– volume: 33
  start-page: 108213
  year: 2020
  ident: 2024061311310930300_JCS257543C50
  article-title: A pushing mechanism for microtubule aster positioning in a large cell type
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2020.108213
  contributor:
    fullname: Meaders
– volume: 27
  start-page: 3235
  year: 2008
  ident: 2024061311310930300_JCS257543C69
  article-title: Dynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly
  publication-title: EMBO J.
  doi: 10.1038/emboj.2008.242
  contributor:
    fullname: Tanenbaum
– volume: 11
  start-page: 3750
  year: 2012
  ident: 2024061311310930300_JCS257543C39
  article-title: End-on microtubule-dynein interactions and pulling-based positioning of microtubule organizing centers
  publication-title: Cell Cycle
  doi: 10.4161/cc.21753
  contributor:
    fullname: Laan
– volume: 94
  start-page: 6228
  year: 1997
  ident: 2024061311310930300_JCS257543C24
  article-title: Assembly and positioning of microtubule asters in microfabricated chambers
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.94.12.6228
  contributor:
    fullname: Holy
– volume: 7
  start-page: 1113
  year: 2005
  ident: 2024061311310930300_JCS257543C45
  article-title: Centering of a radial microtubule array by translocation along microtubules spontaneously nucleated in the cytoplasm
  publication-title: Nat. Cell Biol.
  doi: 10.1038/ncb1332
  contributor:
    fullname: Malikov
– volume: 118
  start-page: 1097
  year: 1992
  ident: 2024061311310930300_JCS257543C74
  article-title: Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.118.5.1097
  contributor:
    fullname: Verde
– start-page: 27
  year: 2017
  ident: 2024061311310930300_JCS257543C81
  publication-title: Handbook of Discrete and Computational Geometry
  doi: 10.1201/9781315119601
  contributor:
    fullname: Fejes Tóth
– volume: 48
  year: 2016
  ident: 2024061311310930300_JCS257543C56
  article-title: The p90 ribosomal S6 kinase 2 specifically affects mitotic progression by regulating the basal level, distribution and stability of mitotic spindles
  publication-title: Exp. Mol. Med.
  doi: 10.1038/emm.2016.61
  contributor:
    fullname: Park
– volume: 9
  start-page: 1150
  year: 2020
  ident: 2024061311310930300_JCS257543C26
  article-title: Managing the oocyte meiotic arrest-lessons from frogs and jellyfish
  publication-title: Cells
  doi: 10.3390/cells9051150
  contributor:
    fullname: Jessus
– volume: 20
  start-page: 188
  year: 1831
  ident: 2024061311310930300_JCS257543C82
  article-title: Untersuchungen über die Eigenschaften der positiven ternären quadratischen Formen von Ludwig August Seeber
  publication-title: Göttingische gelehrte Anzeigen
  contributor:
    fullname: Gauss
– volume: 16
  start-page: 8554
  year: 2020
  ident: 2024061311310930300_JCS257543C31
  article-title: Aster swarming by symmetry breaking of cortical dynein transport and coupling kinesins
  publication-title: Soft Mat.
  doi: 10.1039/D0SM01086C
  contributor:
    fullname: Khetan
– volume: 95
  start-page: 4293
  year: 1998
  ident: 2024061311310930300_JCS257543C66
  article-title: Chromophore-assisted light inactivation and self-organization of microtubules and motors
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.95.8.4293
  contributor:
    fullname: Surrey
– volume: 9
  start-page: 472
  year: 2008
  ident: 2024061311310930300_JCS257543C7
  article-title: Cytoplasmic dynein is involved in the retention of microtubules at the centrosome in interphase cells
  publication-title: Traffic
  doi: 10.1111/j.1600-0854.2007.00698.x
  contributor:
    fullname: Burakov
– volume: 21
  start-page: 4418
  year: 2010
  ident: 2024061311310930300_JCS257543C80
  article-title: Finding the cell center by a balance of dynein and myosin pulling and microtubule pushing: a computational study
  publication-title: Mol. Biol. Cell
  doi: 10.1091/mbc.e10-07-0627
  contributor:
    fullname: Zhu
– volume: 297
  start-page: 235
  year: 2012
  ident: 2024061311310930300_JCS257543C47
  article-title: Cell-cycle control in oocytes and during early embryonic cleavage cycles in ascidians
  publication-title: Int. Rev. Cell Mol. Biol.
  doi: 10.1016/B978-0-12-394308-8.00006-6
  contributor:
    fullname: McDougall
– volume: 351
  start-page: 217
  year: 2011
  ident: 2024061311310930300_JCS257543C67
  article-title: A cell-based model of Nematostella vectensis gastrulation including bottle cell formation, invagination and zippering
  publication-title: Dev. Biol.
  doi: 10.1016/j.ydbio.2010.10.017
  contributor:
    fullname: Tamulonis
– volume: 8
  start-page: 461
  year: 2005
  ident: 2024061311310930300_JCS257543C21
  article-title: Spindle positioning by cortical pulling forces
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2005.03.014
  contributor:
    fullname: Grill
– volume: 9
  start-page: 505
  year: 2020
  ident: 2024061311310930300_JCS257543C49
  article-title: Microtubule-based mechanismS of pronuclear positioning
  publication-title: Cells
  doi: 10.3390/cells9020505
  contributor:
    fullname: Meaders
– volume: 366
  start-page: 569
  year: 2019
  ident: 2024061311310930300_JCS257543C51
  article-title: Toward synthetic cells
  publication-title: Science.
  doi: 10.1126/science.aaz5635
  contributor:
    fullname: Mitchison
– volume: 99
  start-page: 16788
  year: 2002
  ident: 2024061311310930300_JCS257543C16
  article-title: Dynamics of microtubule asters in microfabricated chambers: the role of catastrophes
  publication-title: Proc. Nat. Acad. Sci. USA
  doi: 10.1073/pnas.252407099
  contributor:
    fullname: Faivre-Moskalenko
– volume: 408
  start-page: 297
  year: 2007
  ident: 2024061311310930300_JCS257543C3
  article-title: The selectivity of protein kinase inhibitors: a further update
  publication-title: Biochem. J.
  doi: 10.1042/BJ20070797
  contributor:
    fullname: Bain
– volume: 19
  start-page: 1833
  year: 2009
  ident: 2024061311310930300_JCS257543C18
  article-title: Dynein antagonizes EG5 by crosslinking and sliding antiparallel microtubules
  publication-title: Curr. Biol.
  doi: 10.1016/j.cub.2009.09.025
  contributor:
    fullname: Ferenz
– volume: 36
  start-page: 675
  year: 2007
  ident: 2024061311310930300_JCS257543C35
  article-title: Load-dependent release limits the processive stepping of the tetrameric Eg5 motor
  publication-title: Eur. Biophys. J.
  doi: 10.1007/s00249-007-0134-6
  contributor:
    fullname: Korneev
– volume: 120
  start-page: 923
  year: 1993
  ident: 2024061311310930300_JCS257543C19
  article-title: Flexural rigidity of microtubules and actin filaments measured from thermal fluctuations in shape
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.120.4.923
  contributor:
    fullname: Gittes
– volume: 10
  start-page: 608
  year: 2020
  ident: 2024061311310930300_JCS257543C42
  article-title: Role of p90 ribosomal S6 kinase in long-term synaptic facilitation and enhanced neuronal excitability
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-020-57484-y
  contributor:
    fullname: Liu
– volume: 366
  start-page: 631
  year: 2019
  ident: 2024061311310930300_JCS257543C11
  article-title: Spontaneous emergence of cell-like organization in Xenopus egg extracts
  publication-title: Science
  doi: 10.1126/science.aav7793
  contributor:
    fullname: Cheng
– volume: 401
  start-page: 29
  year: 2007
  ident: 2024061311310930300_JCS257543C59
  article-title: Bi-D1870 is a specific inhibitor of the p90 RSK (ribosomal S6 kinase) isoforms in vitro and in vivo
  publication-title: Biochem. J.
  doi: 10.1042/BJ20061088
  contributor:
    fullname: Sapkota
– volume: 47
  start-page: 363
  year: 2018
  ident: 2024061311310930300_JCS257543C64
  article-title: RSK-MASTL pathway delays meiotic exit in mouse zygotes to ensure paternal chromosome stability
  publication-title: Dev. Cell
  doi: 10.1016/j.devcel.2018.09.011
  contributor:
    fullname: Soeda
SSID ssj0007297
Score 2.442062
Snippet Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia, form...
ABSTRACT Radial microtubule (MT) arrays or asters determine cell geometry in animal cells. Multiple asters interacting with motors, such as those in syncytia,...
SourceID hal
proquest
crossref
SourceType Open Access Repository
Aggregation Database
SubjectTerms Life Sciences
Title Self-organized optimal packing of kinesin-5-driven microtubule asters scales with cell size
URI https://search.proquest.com/docview/2536797398
https://hal.science/hal-03435922
Volume 134
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3da9swEBdrx2AvZZ8s6za0j7egzh-SLT-WsjWUUQZrIbAHI8sSKW2dkdiF9a_fXU6x16yDbi8mKD451v3y0-l0d2LsQxI550wuhcwzI2RRaWG0ViI3kTbOJqm1q2iL42xyKo-majqEFa2yS9pqz17fmlfyP1qFNtArZsn-g2b7TqEBPoN-4QoahuuddPzNXXhB5zJdg-E4h7__JWZWGXsegpnPMar9rBFK1AvktfElBuC1XdVduLHBIgnL8RLU5EKWG_rxx0vo7S9GK31PfNBz9cy15EY9drOe5b8uOjBvycN9SNvxi5DBT75XWKZTeQPMiO87O5gB-V6FRKIj04T87OCXSGLcUqfMzD5PIMbNYaIvR_QqccM4pjIvPf8Gb2YAWnQrsYMlgcRul3vAMUqmw_S13rLfmNX6WENc5YB0CbIlyW6x-1g2EU9aOJwOAUGwzAgH_NLvDsVsQfbj8Nwb5svWDINnN-bwlWFy8ojtBOXwfYLHY3bPNU_YAzpj9OdT9v0mSHgACQ8g4XPPN0HCfwMJJ5BwAglHkHAEAUeQPGOnnz-dHExEOFFD2DSVrfCRrhLj61zDStHZXCsvDVj4WVYpbzyQu6wTY6TJk9qrCsYI7N9au9h4X2nt0udsu5k37gXjkQLxHKRdqmQcmcJbY2IXqcRYrPk4Yu_XA1X-oMIp5Z-qGLF3MIb9DVjrfLL_pcS2KAVLvkiSq3jE3q6HuATyw5cE-M077CTN8iJPC_3yTo_bZQ8HqL5i2-2ic6_BqGyrNyss_AL0GHlO
link.rule.ids 230,314,780,784,885,27924,27925
linkProvider Geneva Foundation for Medical Education and Research
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Self-organized+optimal+packing+of+kinesin-5-driven+microtubule+asters+scales+with+cell+size&rft.jtitle=Journal+of+cell+science&rft.au=Khetan%2C+Neha&rft.au=Pruliere%2C+G%C3%A9rard&rft.au=Hebras%2C+Celine&rft.au=Chenevert%2C+Janet&rft.date=2021-05-15&rft.issn=0021-9533&rft.eissn=1477-9137&rft.volume=134&rft.issue=10&rft_id=info:doi/10.1242%2Fjcs.257543&rft.externalDBID=n%2Fa&rft.externalDocID=10_1242_jcs_257543
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-9533&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-9533&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-9533&client=summon