Proliferation and osteogenic differentiation of human mesenchymal stem cells in PCL/silanated silica composite scaffolds for bone tissue regeneration

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 79; pp. 41 - 51
• Main Authors: Jeon, Hojun, Yun, Seokhwan, Choi, Eunjeong, Kang, Donggu, Park, Keun-ho, Kim, Dongyun, Jin, Songwan, Shim, Jin-Hyung, Yun, Won-Soo, Park, Jongbong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.11.2019; 한국공업화학회
• Subjects: Composite scaffolds; Human mesenchymal stem cells (hMSCs); Poly(ε-caprolactone); Silane-modification; Silica; 화학공학; Poly(ε-caprolactone); Composite scaffolds; Silane-modification; Human mesenchymal stem cells (hMSCs); Silica
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2019.04.050

This illustration is representative of the physical and biological characteristics of the composite scaffold. The composite scaffold, which has been fabricated through three processes, exhibits excellent improved physical properties as well as outstanding osteogenic differentiation. Silanated silica reinforced composite scaffold showed good performance including tensile modulus, water absorption ability, cell initial attachment, proliferation and osteogenic differentiation and have the potential to become implantable materials in hard bone tissue regeneration. [Display omitted] •Composite scaffolds composed of PCL and silanated silica particles were suggested for bone tissue regeneration.•The compsite scaffolds were fabricated by optimizing processing conditions including simple melt-blending method and melt-3D printing process.•The composite scaffolds showed remarkable physical properties and produced significant osteogenic differentiation results. Here, we fabricated composite scaffolds consisting of poly ε-caprolactone (PCL) and silanated silica particles and evaluated the performance of these composite scaffolds as suitable alternatives for bone tissue regeneration including their physical properties (tensile modulus, wettability, and surface roughness) and biological properties (cell proliferation, osteogenic differentiation, Q-PCR, western blotting, and migration assay), by culturing human mesenchymal stem cells. PCL/silanated silica scaffolds had outstanding physical characteristics and biological properties including cell proliferation, osteogenic differentiation, and osteoinduction. This silanated-silica composite has a strong potential as a biomaterial and can be applied to bone tissue applications.