Bioprospecting of the probiotic potential of yeasts isolated from a wine environment

• Published in: Fungal genetics and biology Vol. 164; p. 103767
• Main Authors: Vergara Alvarez, Silvia Cristina, Leiva Alaniz, María José, Mestre Furlani, María Victoria, Vazquez, Fabio, Mancha Agresti, Pamela, Cristina Nally, María, Paola Maturano, Yolanda
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2023
• Subjects: Adhesion properties; Bioprospecting; Biosafety traits; Gastrointestinal resistance; Gastrointestinal Tract; Probiotic; Probiotics; Saccharomyces cerevisiae; Simulated GIT condition; Wine; Wine native yeasts; Yeasts - genetics; Probiotic; Adhesion properties; Wine native yeasts; Gastrointestinal resistance; Biosafety traits; Simulated GIT condition
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/j.fgb.2022.103767

[Display omitted] •Potential Probiotic of 96 native yeasts isolated from wine environment were evaluated.•Biosafety traits assayed, hemolytic, antifungal, urease, allowed to select 66 yeasts.•Fifteen non – Saccharomyces yeasts showed resistant to Gastrointestinal conditions.•The best results were registered by the yeasts of Pichia genus.•Selected yeasts reached high populations after passage by tract gastrointestinal. Autochthonous yeasts of oenological origin are adapted to highly stressful and selective environments, which makes them potential candidates for probiotics. The objective of the present study was to explore the probiotic potential of 96 native yeasts of oenological origin, their biosafety, resistance to gastrointestinal tract conditions and adhesion properties. Regarding biosafety, 66 isolates shown negative hemolytic activity, negative urease activity and susceptibility to 3 or more antifungals. After the gastrointestinal resistance test, 15 isolates were selected that showed growth at different temperatures, tolerance to low pH and the presence of bile salts in in vitro tests. In general, survival after simulated conditions of the gastrointestinal tract was high and more restrictive was the duodenal. The results of the adhesion properties showed highly variable hydrophobicity and a high percentage of autoaggregation at 24 h. The maximum production of biofilm was detected in the Pichia strains. Of a total of 96 yeast strains, 15 non-Saccharomyces yeasts presented suitable properties as probiotic candidates. The native winemaking strains performed better than the reference probiotic strain, Saccharomyces cerevisiae var. boulardii CNCM I-745, which reaffirms that these strains are promising probiotic candidates and further studies are necessary to confirm their probiosis.