Expression of Endoplasmic Reticulum Stress Markers GRP78 and CHOP Induced by Oxidative Stress in Blue Light-Mediated Damage of A2E-Containing Retinal Pigment Epithelium Cells

• Published in: Ophthalmic research Vol. 52; no. 4; pp. 224 - 233
• Main Authors: Feng, Jingyang, Chen, Xunjie, Sun, Xiangjun, Wang, Fenghua, Sun, Xiaodong
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2014
• Subjects: Biomarkers - metabolism; Blotting, Western; Cell Line; Cell Survival; Endoplasmic Reticulum Stress - physiology; Flow Cytometry; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Humans; Light; Microscopy, Confocal; Original Paper; Oxidative Stress; Reactive Oxygen Species - metabolism; Real-Time Polymerase Chain Reaction; Retinal Pigment Epithelium - drug effects; Retinal Pigment Epithelium - metabolism; Retinal Pigment Epithelium - radiation effects; Retinoids - toxicity; RNA, Messenger - genetics; Transcription Factor CHOP - genetics; Transcription Factor CHOP - metabolism; Transfection; Oxidative stress; Glucose-related protein 78; N-retinylidene-N-retinylethanolamine; Retinal pigment epithelium; Endoplasmic reticulum stress

Aims: Age-related lipofuscin N-retinylidene-N-retinylethanolamine (A2E) accumulated in human retinal pigment epithelium (RPE) cells confers susceptibility to blue light-mediated damage, which represents one pathogenesis of age-related macular degeneration. This study investigated the expression of 2...