Rapid genotyping of 508G>A (rs3745635) and 1067T>A (rs3894326) of FUT3 by a duplex Eprobe‐mediated melting curve analysis

• Published in: Vox sanguinis Vol. 117; no. 5; pp. 741 - 745
• Main Authors: Soejima, Mikiko, Koda, Yoshiro
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.2022; S. Karger AG
• Subjects: Alleles; Blood groups; Deoxyribonucleic acid; DNA; Eprobe; FUT3; Genotyping; Lewis‐negative allele; Melting; Melting curve; melting curve analysis; Nucleotide sequence; Nucleotides; Phenotypes; Polymerase chain reaction; Polymorphism; Restriction fragment length polymorphism; rs3745635; rs3894326; Sequence analysis; Single-nucleotide polymorphism; Lewis-negative allele; melting curve analysis; rs3745635; Eprobe; FUT3; rs3894326

Background and Objectives Lewis histo‐blood group phenotypes are regulated by the action of FUT3‐encoded α(1,3/1,4)fucosyltransferase and FUT2‐encoded α(1,2)fucosyltransferase. Since Lewis phenotypes are suggested to be associated with various clinical conditions, a method for large‐scale FUT3 genot...