PVA/PMMA polymer blended composite electrospun nanofibers mat and their potential use as an anti‐biofilm product

Bacterial infections have increased dramatically due to microbial biofilm formation resulting in chronic pathological conditions in human subjects. Microbial biofilm causes poor drug penetration and antibiotic resistance, which has made site‐specific sustained drug delivery the most appropriate opti...

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Bibliographic Details
Published inJournal of applied polymer science Vol. 138; no. 18
Main Authors Singh, Manvi, Chauhan, Deepika, Das, Ayan K., Iqbal, Zeenat, Solanki, Pratima R.
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 10.05.2021
Wiley Subscription Services, Inc
Subjects
Antibiotics
Bacterial diseases
biofilm
Biofilms
drug delivery systems
E coli
electrospinning
Materials science
Microorganisms
Morphology
Nanofibers
Penetration resistance
Polymers
Polymethyl methacrylate
Polyvinyl alcohol
Sustained release
Thermal analysis
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Summary:Bacterial infections have increased dramatically due to microbial biofilm formation resulting in chronic pathological conditions in human subjects. Microbial biofilm causes poor drug penetration and antibiotic resistance, which has made site‐specific sustained drug delivery the most appropriate option. Our work entails fabrication of ciprofloxacin hydrochloride (CPX) loaded nanofibers using polyvinyl alcohol (PVA) and poly(meth) methacrylate (PMMA) employing electrospinning method to form PVA:PMMA:CPX nanofibers mat. These nanofibers mat were optimized, characterized, and further subjected to anti‐biofilm activity. Microscopic images revealed average nanofibers diameter of 243 ± 80 nm with smooth surface morphology. Analytical graphs and thermal analysis confirmed drug encapsulation and drug‐polymer compatibility. In vitro studies demonstrated sustained release of CPX for 22 days displaying Hixon Crowell and two‐stage desorption kinetics. Anti‐biofilm activity showed zones of inhibition 3.0 ± 0.5 cm, 2.8 ± 0.1 cm, 2.9 ± 0.2 cm for Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, respectively which was well above the minimum inhibitory concentration levels of the bacteria forming biofilm. Conclusively, these nanofibers mat have potential to be used as an anti‐biofilm product.
Bibliography:Funding information
Department of Biotechnology, Ministry of science and technology, India
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:0021-8995
1097-4628
DOI:10.1002/app.50340