Automatic 3D alignment of projection images of randomly oriented objects

• Published in: Ultramicroscopy Vol. 52; no. 2; pp. 141 - 156
• Main Authors: Farrow, Neil A., Ottensmeyer, F.Peter
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.11.1993; Elsevier Science
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Simulation; Projection; Biological macromolecule; Density distribution; Experimental study; Image; Electron microscopy; Micrography

Reconstruction of the 3D density distribution of macromolecular structures from their 2D projection images or electron micrographs requires knowledge of the relative orientations of the 2D projections. Using a technique that combines the principles of the common axis theorem and quaternion mathemati...