Identification and Localization of a Serotypic Neutralization Determinant on the VP2 Protein of Bluetongue Virus 13

• Published in: Virology (New York, N.Y.) Vol. 195; no. 2; pp. 859 - 862
• Main Authors: Hwang, Guang-Yuh, Li, Joseph K.-K.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.08.1993; Elsevier
• Subjects: Amino Acid Sequence; Animals; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; Biological and medical sciences; Blotting, Western; Bluetongue virus - classification; Bluetongue virus - immunology; bluetongue virus 13; Capsid - immunology; Capsid Proteins; Cell Line; CONFORMATION ANIMALE; Cricetinae; Epitopes - analysis; Epitopes - immunology; Fundamental and applied biological sciences. Psychology; Mice; Microbiology; Molecular Sequence Data; MORFOLOGIA ANIMAL; Morphology, structure, chemical composition, physicochemical properties; Neutralization Tests; ORBIVIRUS; PEPTIDE; PEPTIDOS; PROTEINAS; PROTEINE; REACCION ANTIGENO-ANTICUERPO; REACTION ANTIGENE ANTICORPS; SEROTIPOS; SEROTYPE; Serotyping; Virology; VIRUS DE LOS ANIMALES; VIRUS DES ANIMAUX; Antigenic determinant; Peptides; Monoclonal antibody; Reoviridae; Virus; Antigenicity; Serotype specificity; Orbivirus; Capsid; Localization; Virus surface; Neutralization; Bluetongue virus

We have used a serotype-specific monoclonal antibody to locate a neutralization epitope on the outer capsid protein, VP2, of the bluetongue virus 13. This surface-accessible region of the virion was recognized by a monoclonal antibody, D24.15, which exhibited serotype-specific neutralizing activity...