Separation of single component of EPA and DHA from fish oil using silver ion modified molecular sieve 13X under supercritical condition
The importance of polyunsaturated fatty acids (PUFAs) in human nutrition and disease prevention was demonstrated four decades ago. EPA and DHA are important PUFAs. Separation of EPA and DHA has been carried out under supercritical condition by using a silver ion modified molecular sieve 13X (MS13X)....
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| Published in | Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 14; no. 5; pp. 639 - 643 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
01.09.2008
한국공업화학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1226-086X 1876-794X |
| DOI | 10.1016/j.jiec.2008.04.011 |
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| Abstract | The importance of polyunsaturated fatty acids (PUFAs) in human nutrition and disease prevention was demonstrated four decades ago. EPA and DHA are important PUFAs. Separation of EPA and DHA has been carried out under supercritical condition by using a silver ion modified molecular sieve 13X (MS13X). MS13X was modified by silver nitrate, and the modification reaction was optimized by stir model, the concentration of silver nitrate and temperature. It was found that the optimized reaction conditions are stirring with nitrogen bubbling, 0.1
mol/l silver nitrate and 20
°C in the dark. Results indicated that 1
g MS13X could be bound by 7.4
mg silver ion, namely 12.4% sodium of MS13X was exchanged. The modified MS13X (called SN13X) displays maximum adsorption capacity of EPA and DHA at 40
°C, with 37.4
mg/g and 24.2
mg/g, respectively. SN13X was packed in the column (20
cm
×
1
cm) under supercritical fluid apparatus, and adsorption, washing and elution of EPA and DHA were carried out by gradient pressure. In this study, the purity of EPA and DHA were enhanced to 88.4% and 84.3% from 20.4% and 12.7%, with recovery of 45.8% and 39.2%, respectively. |
|---|---|
| AbstractList | The importance of polyunsaturated fatty acids (PUFAs) in human nutrition and disease prevention was demonstrated four decades ago. EPA and DHA are important PUFAs. Separation of EPA and DHA has been carried out under supercritical condition by using a silver ion modified molecular sieve 13X (MS13X). MS13X was modified by silver nitrate, and the modification reaction was optimized by stir model, the concentration of silver nitrate and temperature. It was found that the optimized reaction conditions are stirring with nitrogen bubbling, 0.1 mol/l silver nitrate and 20 8C in the dark. Results indicated that 1 g MS13X could be bound by 7.4 mg silver ion, namely 12.4% sodium of MS13X was exchanged. The modified MS13X (called SN13X) displays maximum adsorption capacity of EPA and DHA at 40 8C, with 37.4 mg/g and 24.2 mg/g, respectively. SN13X was packed in the column (20 cm 1 cm) under supercritical fluid apparatus, and adsorption, washing and elution of EPA and DHAwere carried out by gradient pressure. In this study, the purity of EPA and DHAwere enhanced to 88.4% and 84.3% from 20.4% and 12.7%, with recovery of 45.8% and 39.2%, respectively. KCI Citation Count: 8 The importance of polyunsaturated fatty acids (PUFAs) in human nutrition and disease prevention was demonstrated four decades ago. EPA and DHA are important PUFAs. Separation of EPA and DHA has been carried out under supercritical condition by using a silver ion modified molecular sieve 13X (MS13X). MS13X was modified by silver nitrate, and the modification reaction was optimized by stir model, the concentration of silver nitrate and temperature. It was found that the optimized reaction conditions are stirring with nitrogen bubbling, 0.1 mol/l silver nitrate and 20 °C in the dark. Results indicated that 1 g MS13X could be bound by 7.4 mg silver ion, namely 12.4% sodium of MS13X was exchanged. The modified MS13X (called SN13X) displays maximum adsorption capacity of EPA and DHA at 40 °C, with 37.4 mg/g and 24.2 mg/g, respectively. SN13X was packed in the column (20 cm × 1 cm) under supercritical fluid apparatus, and adsorption, washing and elution of EPA and DHA were carried out by gradient pressure. In this study, the purity of EPA and DHA were enhanced to 88.4% and 84.3% from 20.4% and 12.7%, with recovery of 45.8% and 39.2%, respectively. |
| Author | Hao, Liu-Ping Hur, Byung-Ki Cao, Xue-Jun |
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| CitedBy_id | crossref_primary_10_1016_j_chroma_2020_461326 crossref_primary_10_1007_s12010_014_1159_9 crossref_primary_10_1016_j_foodcont_2021_107864 crossref_primary_10_1016_j_jiec_2011_09_008 crossref_primary_10_1016_j_jiec_2009_08_008 crossref_primary_10_1016_j_molliq_2017_07_013 crossref_primary_10_1002_apj_1779 |
| Cites_doi | 10.1016/S0896-8446(00)00072-3 10.1016/S0022-0728(01)00678-7 10.1016/S0896-8446(99)00038-8 10.1016/S0021-9673(01)95827-3 10.1007/BF02541694 10.2331/suisan.59.1429 10.1007/BF02667433 10.1021/cr60282a005 10.1111/j.1753-4887.1986.tb07603.x 10.1016/S0896-8446(98)00100-4 10.1093/chromsci/27.6.318 10.1016/S0021-9673(00)90097-9 10.1021/ac00291a019 10.2331/suisan.44.927 |
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| Title | Separation of single component of EPA and DHA from fish oil using silver ion modified molecular sieve 13X under supercritical condition |
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