Zygote and vegetative cell wall proteins in Chlamydomonas reinhardtii share a common epitope, (SerPro) X

• Published in: Plant science (Limerick) Vol. 83; no. 1; pp. 65 - 76
• Main Authors: Woessner, Jeffrey P., Goodenough, Ursula W.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1992; Elsevier Science
• Subjects: Biological and medical sciences; Cell biochemistry; Cell physiology; cell wall proteins; Chlamydomonas reinhardtii; Fundamental and applied biological sciences. Psychology; immunoprecipitation; in vitro translation; Plant physiology and development; vegetative cells; zygotes; zygotes; in vitro translation; cell wall proteins; Chlamydomonas reinhardtii; immunoprecipitation; vegetative cells; Proteins; Antigenic determinant; Zygote; Algae; Chlorophycophyta; Vegetative apparatus; Translating; Cell wall; Thallophyta; Immunological method
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/0168-9452(92)90063-R

Polyclonal antibodies directed against a (SerPro) 10 oligopeptide and a ß-galactosidase/zygote-wall-gene fusion protein were used in in vitro translation/immunoprecipitation experiments to show that (SerPro) x is a common motif in proteins which assemble into either the zygotic or the vegetative/gametic cell walls of Chlamydomonas reinhardtii. There are at least five, and possibly as many as ten different zygote-specific proteins displaying (SerPro) x -like epitopes. Zygote-specific cDNAs corresponding to two of these proteins have been previously characterized and shown to encode several regions of (SerPro) ranging from 2 to 9 repeats. In addition, the (SerPro) 10 antibody recognizes five vegetative/gamete-specific proteins from cells induced for wall synthesis. One of these five corresponds to the vegetative/gamete-wall glycoprotein GP2, as confirmed by immunoprecipitation with a polyclonal antibody to purified GP2. The GP2 antibody detects epitopes other than (SerPro) x and also immunoprecipitates a novel zygote-specific protein.