Transformation and regeneration of Nicotiana edwardsonii

• Published in: Plant science (Limerick) Vol. 64; no. 1; pp. 67 - 78
• Main Authors: Kiernan, J.M., Goldberg, K.-B., Young, M.J., Schoelz, J.E., Shepherd, R.J.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1989; Elsevier Science
• Subjects: Agrobacterium tumefaciens; Agronomy. Soil science and plant productions; Biological and medical sciences; caulimoviruses; Classical genetics, quantitative genetics, hybrids; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; In vitro culture; Nicotiana clevelandii; Nicotiana edwardsonii; Nicotiana glutinosa; Pteridophyta, spermatophyta; tissue culture; Vegetals; tissue culture; MS; NPT II; Agrobacterium tumefaciens; IAA; bp; FMV; NAA; caulimoviruses; BAP; Nicotiana clevelandii; Nicotiana edwardsonii; CaMV; 2iP; Nicotiana glutinosa; Culture medium; Nicotiana; Genetic transformation; Genetic marker; Tissue culture; Regeneration; Antibiotic; Sensitivity resistance; Dicotyledones; Angiospermae; Immunoblotting assay; Bacteria; Spermatophyta; Transgenic plant; Rhizobiaceae; Solanaceae; Southern blotting; Shoot

Several previously developed media for regeneration of Nicotiana species were evaluated for their ability to regenerate shoots of Nicotiana edwardsonii, a fertile amphidiploid of N. glutinosa × N. clevelandii. Two media were superior to the others, producing shoots within 3–4 weeks. Roots were easil...