Transformation and regeneration of Nicotiana edwardsonii
Several previously developed media for regeneration of Nicotiana species were evaluated for their ability to regenerate shoots of Nicotiana edwardsonii, a fertile amphidiploid of N. glutinosa × N. clevelandii. Two media were superior to the others, producing shoots within 3–4 weeks. Roots were easil...
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Published in | Plant science (Limerick) Vol. 64; no. 1; pp. 67 - 78 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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Shannon
Elsevier Ireland Ltd
1989
Elsevier Science |
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Abstract | Several previously developed media for regeneration of
Nicotiana species were evaluated for their ability to regenerate shoots of
Nicotiana edwardsonii, a fertile amphidiploid of
N. glutinosa ×
N. clevelandii. Two media were superior to the others, producing shoots within 3–4 weeks. Roots were easily regenerated on a simple rooting medium. Ti plasmid-
Agrobacterium tumefaciens mediated DNA transfer was used to genetically transform
N. edwardsonii with gene VI of strains D4 and CM1841 of cauliflower mosaic virus (CaMV) and strain Sc3 of figwort mosaic virus (FMV). When using gene vectors with the neomycin phosphotransferase type II gene and antibiotics kanamycin or G418, a related antibiotic, in a positive selection procedure, a marked inhibition of shoot formation was observed. Consequently, it was necessary to use much lower concentrations of the antibiotics in the selection media to obtain transgenic plants than are used for other species such as
N. tabacum and
Datura innoxia. Insertion of the desired caulimoviral genes was demonstrated by the presence of the appropriate DNA sequences and the corresponding protein products. The level of expression of the inserted genes is briefly compared to
D. innoxia transformed with the same genes. The authors believe this to be a first report of the transformation and regeneration of
N. edwardsonii, a widely used host of plant viruses. |
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AbstractList | Several previously developed media for regeneration of
Nicotiana species were evaluated for their ability to regenerate shoots of
Nicotiana edwardsonii, a fertile amphidiploid of
N. glutinosa ×
N. clevelandii. Two media were superior to the others, producing shoots within 3–4 weeks. Roots were easily regenerated on a simple rooting medium. Ti plasmid-
Agrobacterium tumefaciens mediated DNA transfer was used to genetically transform
N. edwardsonii with gene VI of strains D4 and CM1841 of cauliflower mosaic virus (CaMV) and strain Sc3 of figwort mosaic virus (FMV). When using gene vectors with the neomycin phosphotransferase type II gene and antibiotics kanamycin or G418, a related antibiotic, in a positive selection procedure, a marked inhibition of shoot formation was observed. Consequently, it was necessary to use much lower concentrations of the antibiotics in the selection media to obtain transgenic plants than are used for other species such as
N. tabacum and
Datura innoxia. Insertion of the desired caulimoviral genes was demonstrated by the presence of the appropriate DNA sequences and the corresponding protein products. The level of expression of the inserted genes is briefly compared to
D. innoxia transformed with the same genes. The authors believe this to be a first report of the transformation and regeneration of
N. edwardsonii, a widely used host of plant viruses. |
Author | Kiernan, J.M. Shepherd, R.J. Schoelz, J.E. Goldberg, K.-B. Young, M.J. |
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Cites_doi | 10.1002/j.1460-2075.1985.tb03626.x 10.1094/Phyto-77-1668 10.1016/0042-6822(87)90218-2 10.1093/nar/15.20.8451 10.1038/325177a0 10.1111/j.1399-3054.1962.tb08052.x 10.1016/0378-1119(87)90359-3 10.1038/227680a0 10.1016/0042-6822(88)90391-1 10.1007/BF00020325 10.1093/nar/9.12.2871 10.1126/science.227.4691.1229 10.1099/0022-1317-68-12-3217 10.1016/0014-4827(68)90403-5 10.1094/Phyto-76-451 |
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Keywords | tissue culture MS NPT II Agrobacterium tumefaciens IAA bp FMV NAA caulimoviruses BAP Nicotiana clevelandii Nicotiana edwardsonii CaMV 2iP Nicotiana glutinosa Culture medium Nicotiana Genetic transformation Genetic marker Tissue culture Regeneration Antibiotic Sensitivity resistance Dicotyledones Angiospermae Immunoblotting assay Bacteria Spermatophyta Transgenic plant Rhizobiaceae Solanaceae Southern blotting Shoot |
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References | Young, Daubert, Shepherd (BIB19) 1987; 158 Gamborg, Miller, Ojima (BIB9) 1968; 50 Schoelz, Shepherd, Richins (BIB16) 1986; 76 Baughman, Jacobs, Howell (BIB28) 1988; 85 Schoelz, Shepherd (BIB2) 1988; 162 Christie (BIB1) 1969; 53 Gardner, Howarth, Hahn, Brown-Luedi, Shepherd, Messing (BIB15) 1981; 9 Shepherd, Goldberg, Kiernan, Gowda, Schoelz, Young, Richins (BIB27) 1988 Towbin, Staehelin, Gordon (BIB21) 1979; 76 Kiernan, Goldberg, Schoelz, Gowda, Shepherd (BIB7) 1988; 78 Hughes (BIB11) 1983; Vol. 1 Malmburg, Messing, Sussex (BIB10) 1985 Aviv, Galun (BIB25) 1986; 7 Maniatis, Fritsch, Sambrook (BIB12) 1982 Schoelz, Shepherd, Daubert (BIB4) 1987 Horsch, Fry, Hoffman, Eichholtz, Rogers, Fraley (BIB6) 1985; 227 Goldberg, Kiernan, Gowda, Schoelz, Shepherd (BIB5) 1988; 78 Richins, Scholthof, Shepherd (BIB13) 1987; 15 Young, Shewmaker, Daubert, Shepherd (BIB22) 1987; 68 Laemmli (BIB20) 1970; 227 Schardl, Byrd, Benzion, Altschuler, Hildebrand, Hunt (BIB17) 1987; 61 Flick, Evans, Sharp (BIB24) 1983; Vol. 1 Murashige, Skoog (BIB8) 1962; 15 Grimsley, Hohn, Davies, Hohn (BIB18) 1987; 325 Helgeson (BIB23) 1979 Goldberg, Young, Schoelz, Kiernan, Shepherd (BIB26) 1987; 77 Shepherd, Richins, Duffus, Handley (BIB3) 1987; 77 An, Watson, Stachel, Gordon, Nester (BIB14) 1985; 4 An (10.1016/0168-9452(89)90153-2_BIB14) 1985; 4 Baughman (10.1016/0168-9452(89)90153-2_BIB28) 1988; 85 Gardner (10.1016/0168-9452(89)90153-2_BIB15) 1981; 9 Schoelz (10.1016/0168-9452(89)90153-2_BIB16) 1986; 76 Horsch (10.1016/0168-9452(89)90153-2_BIB6) 1985; 227 Young (10.1016/0168-9452(89)90153-2_BIB19) 1987; 158 Flick (10.1016/0168-9452(89)90153-2_BIB24) 1983; Vol. 1 Aviv (10.1016/0168-9452(89)90153-2_BIB25) 1986; 7 Young (10.1016/0168-9452(89)90153-2_BIB22) 1987; 68 Shepherd (10.1016/0168-9452(89)90153-2_BIB3) 1987; 77 Christie (10.1016/0168-9452(89)90153-2_BIB1) 1969; 53 Helgeson (10.1016/0168-9452(89)90153-2_BIB23) 1979 Schoelz (10.1016/0168-9452(89)90153-2_BIB4) 1987 Goldberg (10.1016/0168-9452(89)90153-2_BIB5) 1988; 78 Laemmli (10.1016/0168-9452(89)90153-2_BIB20) 1970; 227 Maniatis (10.1016/0168-9452(89)90153-2_BIB12) 1982 Schardl (10.1016/0168-9452(89)90153-2_BIB17) 1987; 61 Kiernan (10.1016/0168-9452(89)90153-2_BIB7) 1988; 78 Richins (10.1016/0168-9452(89)90153-2_BIB13) 1987; 15 Schoelz (10.1016/0168-9452(89)90153-2_BIB2) 1988; 162 Malmburg (10.1016/0168-9452(89)90153-2_BIB10) 1985 Goldberg (10.1016/0168-9452(89)90153-2_BIB26) 1987; 77 Murashige (10.1016/0168-9452(89)90153-2_BIB8) 1962; 15 Towbin (10.1016/0168-9452(89)90153-2_BIB21) 1979; 76 Gamborg (10.1016/0168-9452(89)90153-2_BIB9) 1968; 50 Hughes (10.1016/0168-9452(89)90153-2_BIB11) 1983; Vol. 1 Grimsley (10.1016/0168-9452(89)90153-2_BIB18) 1987; 325 Shepherd (10.1016/0168-9452(89)90153-2_BIB27) 1988 |
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DNA sequence within gene VI contributor: fullname: Schoelz – volume: 4 start-page: 277 year: 1985 ident: 10.1016/0168-9452(89)90153-2_BIB14 article-title: New cloning vehicles for transformation of higher plants publication-title: EMBO J. doi: 10.1002/j.1460-2075.1985.tb03626.x contributor: fullname: An – volume: 78 start-page: 1600 year: 1988 ident: 10.1016/0168-9452(89)90153-2_BIB7 article-title: Ti plasmid transformation and regeneration of Nicotiana edwardsonii — a useful host for many plant viruses publication-title: Phytopathology contributor: fullname: Kiernan – volume: 77 start-page: 1668 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB3 article-title: Figwort mosaic virus: Properties of the virus and its adaptation to a new host publication-title: Phytopathology doi: 10.1094/Phyto-77-1668 contributor: fullname: Shepherd – volume: 158 start-page: 444 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB19 article-title: Gene I products of cauliflower mosaic virus detected in extracts of infected tissue publication-title: Virology doi: 10.1016/0042-6822(87)90218-2 contributor: fullname: Young – volume: 15 start-page: 8451 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB13 article-title: Sequence of figwort mosaic virus DNA (caulimovirus group) publication-title: Nucl. Acid. Res. doi: 10.1093/nar/15.20.8451 contributor: fullname: Richins – volume: 325 start-page: 177 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB18 article-title: Agrobacterium-mediated delivery of infectious maize streak virus into maize plants publication-title: Nature (Lond.) doi: 10.1038/325177a0 contributor: fullname: Grimsley – volume: Vol. 1 start-page: 442 year: 1983 ident: 10.1016/0168-9452(89)90153-2_BIB11 article-title: Selection for herbicide resistance contributor: fullname: Hughes – start-page: 52 year: 1979 ident: 10.1016/0168-9452(89)90153-2_BIB23 article-title: Tissue and cell-suspension culture contributor: fullname: Helgeson – volume: 15 start-page: 485 year: 1962 ident: 10.1016/0168-9452(89)90153-2_BIB8 article-title: A revised medium for rapid growth and bioassays with tobacco tissue cultures publication-title: Physiol. Planta. doi: 10.1111/j.1399-3054.1962.tb08052.x contributor: fullname: Murashige – start-page: 131 year: 1988 ident: 10.1016/0168-9452(89)90153-2_BIB27 article-title: Genomic changes during host adaptation by caulimoviruses contributor: fullname: Shepherd – year: 1982 ident: 10.1016/0168-9452(89)90153-2_BIB12 contributor: fullname: Maniatis – volume: 61 start-page: 1 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB17 article-title: Design and construction of a versatile system for the expression of foreign genes in plants publication-title: Gene doi: 10.1016/0378-1119(87)90359-3 contributor: fullname: Schardl – volume: 227 start-page: 680 year: 1970 ident: 10.1016/0168-9452(89)90153-2_BIB20 article-title: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 publication-title: Nature (Lond.) doi: 10.1038/227680a0 contributor: fullname: Laemmli – volume: 162 start-page: 30 year: 1988 ident: 10.1016/0168-9452(89)90153-2_BIB2 article-title: Host range control of cauliflower mosaic virus publication-title: Virology doi: 10.1016/0042-6822(88)90391-1 contributor: fullname: Schoelz – volume: 7 start-page: 411 year: 1986 ident: 10.1016/0168-9452(89)90153-2_BIB25 article-title: Restoration of male fertile Nicotiana by fusion of protoplasts derived from two different cytoplasmic male-sterile cybrids publication-title: Plant Mol. Biol. doi: 10.1007/BF00020325 contributor: fullname: Aviv – volume: 9 start-page: 2871 year: 1981 ident: 10.1016/0168-9452(89)90153-2_BIB15 article-title: The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13 mp7 shotgun sequencing publication-title: Nucl. Acid Res. doi: 10.1093/nar/9.12.2871 contributor: fullname: Gardner – volume: 227 start-page: 1229 year: 1985 ident: 10.1016/0168-9452(89)90153-2_BIB6 article-title: A simple and general method for transferring genes into plants publication-title: Science doi: 10.1126/science.227.4691.1229 contributor: fullname: Horsch – volume: 68 start-page: 3217 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB22 article-title: Expression of cauliflower mosaic virus proteins from an integrated form of the viral genome publication-title: J. Gen. Virol. doi: 10.1099/0022-1317-68-12-3217 contributor: fullname: Young – volume: Vol. 1 start-page: 13 year: 1983 ident: 10.1016/0168-9452(89)90153-2_BIB24 article-title: Organogenesis contributor: fullname: Flick – volume: 50 start-page: 151 year: 1968 ident: 10.1016/0168-9452(89)90153-2_BIB9 article-title: Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells publication-title: Exp. Cell Res. doi: 10.1016/0014-4827(68)90403-5 contributor: fullname: Gamborg – volume: 78 start-page: 1517 year: 1988 ident: 10.1016/0168-9452(89)90153-2_BIB5 article-title: Effects of genes I and VI of two caulimoviruses in transgenic plants publication-title: Phytopathology contributor: fullname: Goldberg – volume: 77 start-page: 1704 year: 1987 ident: 10.1016/0168-9452(89)90153-2_BIB26 article-title: Single gene of CaMV induces disease publication-title: Phytopathology contributor: fullname: Goldberg – start-page: 106 year: 1985 ident: 10.1016/0168-9452(89)90153-2_BIB10 publication-title: Molecular Biology of Plants contributor: fullname: Malmburg – volume: 76 start-page: 451 year: 1986 ident: 10.1016/0168-9452(89)90153-2_BIB16 article-title: Properties of an unusual strain of cauliflower mosaic virus publication-title: Phytopathology doi: 10.1094/Phyto-76-451 contributor: fullname: Schoelz – volume: 76 start-page: 4350 year: 1979 ident: 10.1016/0168-9452(89)90153-2_BIB21 article-title: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications contributor: fullname: Towbin |
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Snippet | Several previously developed media for regeneration of
Nicotiana species were evaluated for their ability to regenerate shoots of
Nicotiana edwardsonii, a... |
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SubjectTerms | Agrobacterium tumefaciens Agronomy. Soil science and plant productions Biological and medical sciences caulimoviruses Classical genetics, quantitative genetics, hybrids Economic plant physiology Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution In vitro culture Nicotiana clevelandii Nicotiana edwardsonii Nicotiana glutinosa Pteridophyta, spermatophyta tissue culture Vegetals |
Title | Transformation and regeneration of Nicotiana edwardsonii |
URI | https://dx.doi.org/10.1016/0168-9452(89)90153-2 |
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