Protein crystallography with non-detergent sulfobetaines

The use in protein crystal growth of the newly described non-detergent solubilizers/stabilizers sulfobetaines (NDSB) has been investigated with crystallographic methods. In the classic model of egg white lysozyme crystallization by NaCl, NDSB increased the protein solubility and the crystal growth r...

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Bibliographic Details
Published inJournal of crystal growth Vol. 168; no. 1; pp. 150 - 154
Main Authors Vuillard, Laurent, Baalbaki, Bassem, Lehmann, Mogens, Nørager, Sofie, Legrand, Pierre, Roth, Michel
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 01.10.1996
Elsevier
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
In solution. Condensed state. Thin layers
Molecular biophysics
Physico-chemical properties of biomolecules
Crystal growth
Ferrosulfoprotein
Betaine
Crystallization
Desulfovibrio gigas
Solubility
Bacteria
Ferredoxin
X ray diffraction
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Summary:The use in protein crystal growth of the newly described non-detergent solubilizers/stabilizers sulfobetaines (NDSB) has been investigated with crystallographic methods. In the classic model of egg white lysozyme crystallization by NaCl, NDSB increased the protein solubility and the crystal growth rate without influencing the quality of crystals as shown by Wilson plots. Sulphur anomalous X-ray scattering was used to show the absence of any bound NDSB in the crystals. A practical example of the use of NDSB is given for Desulfovibrio Gigas type II ferrodoxin, where NDSB contributed to reduction in crystal twinning and resulted in the growth of a new crystal form. With their unique combination of solubilizing and stabilizing properties, the NDSB molecules might prove to be valuable additives for protein crystal growth.
ISSN:0022-0248
1873-5002
DOI:10.1016/0022-0248(96)00348-X