The utility of Y-STR profiling in four-, six- and eight-day postcoital vaginal swabs
Y-STR profiles using the Promega PowerPlex® Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from female partners of 11 couples. At four days postcoitus, full composite profiles (where swabs yielded confirmed alleles at all 11 loci) were obtai...
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Published in | Medicine, science, and the law Vol. 52; no. 2; pp. 81 - 88 |
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Main Authors | , |
Format | Journal Article |
Language | English |
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London, England
SAGE Publications
01.04.2012
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Abstract | Y-STR profiles using the Promega PowerPlex® Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from female partners of 11 couples. At four days postcoitus, full composite profiles (where swabs yielded confirmed alleles at all 11 loci) were obtained for five of the 11 couples and 78% of all possible alleles summed for all couples were confirmed (able to be duplicated in different swabs). Results for composite profiles for all couples taken at six days after intercourse showed that 53% of all alleles summed for all couples were confirmed. Only one couple yielded a full composite profile at six days after intercourse. Composite profiles from swabs taken at eight days after intercourse for all couples confirmed only 44% of all possible alleles summed for all couples. At eight days postcoitus, no couple yielded a full composite profile and the largest number of confirmed alleles for any couple was eight. However, one of 44 individual swabs taken from all couples combined at eight days postcoitus yielded a 10-locus profile. Composite partial profiles from the eight-day postcoital set with confirmed results at a minimum of five loci (8 of 11 couples) yielded haplotype frequencies from 0.000323 to 0.125862 using the Y chromosome haplotype reference database, suggesting that meaningful Y-STR information can still be obtained at much extended postcoital intervals. |
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AbstractList | Y-STR profiles using the Promega PowerPlex(®) Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from female partners of 11 couples. At four days postcoitus, full composite profiles (where swabs yielded confirmed alleles at all 11 loci) were obtained for five of the 11 couples and 78% of all possible alleles summed for all couples were confirmed (able to be duplicated in different swabs). Results for composite profiles for all couples taken at six days after intercourse showed that 53% of all alleles summed for all couples were confirmed. Only one couple yielded a full composite profile at six days after intercourse. Composite profiles from swabs taken at eight days after intercourse for all couples confirmed only 44% of all possible alleles summed for all couples. At eight days postcoitus, no couple yielded a full composite profile and the largest number of confirmed alleles for any couple was eight. However, one of 44 individual swabs taken from all couples combined at eight days postcoitus yielded a 10-locus profile. Composite partial profiles from the eight-day postcoital set with confirmed results at a minimum of five loci (8 of 11 couples) yielded haplotype frequencies from 0.000323 to 0.125862 using the Y chromosome haplotype reference database, suggesting that meaningful Y-STR information can still be obtained at much extended postcoital intervals. Y-STR profiles using the Promega PowerPlex® Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from female partners of 11 couples. At four days postcoitus, full composite profiles (where swabs yielded confirmed alleles at all 11 loci) were obtained for five of the 11 couples and 78% of all possible alleles summed for all couples were confirmed (able to be duplicated in different swabs). Results for composite profiles for all couples taken at six days after intercourse showed that 53% of all alleles summed for all couples were confirmed. Only one couple yielded a full composite profile at six days after intercourse. Composite profiles from swabs taken at eight days after intercourse for all couples confirmed only 44% of all possible alleles summed for all couples. At eight days postcoitus, no couple yielded a full composite profile and the largest number of confirmed alleles for any couple was eight. However, one of 44 individual swabs taken from all couples combined at eight days postcoitus yielded a 10-locus profile. Composite partial profiles from the eight-day postcoital set with confirmed results at a minimum of five loci (8 of 11 couples) yielded haplotype frequencies from 0.000323 to 0.125862 using the Y chromosome haplotype reference database, suggesting that meaningful Y-STR information can still be obtained at much extended postcoital intervals. |
Author | Quarino, Lawrence Kishbaugh, Janine |
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References | Davies, Wilson 1974; 3 Silverman, Silverman 1978; 240 Sibille, Duverneuil, Lorin de la Grandmaison 2002; 125 Johnson, Giles, Warren, Floyd, Staub 2005; 50 Wilson 1974; 19 Stein, Cohen 1950; 1 Hall, Ballantyne 2003; 136 Walsh, Metzger, Higuchi 1991; 10 Randall 1987; 32 Morrison 1972; 48 Rupp 1969; 14 Collins, Rao, Hayworth 1994; 39 Nicklas, Buel 2003; 48 Mayntz-Press, Ballantyne 2008; 53 Norris, Manning, Linke, Ferrance, Landers 2007; 52 Walsh PS (bibr-MSL-11-014C10) 1991; 10 Rupp JC (bibr-MSL-11-014C8) 1969; 14 bibr-MSL-11-014C9 bibr-MSL-11-014C7 bibr-MSL-11-014C6 bibr-MSL-11-014C5 bibr-MSL-11-014C4 bibr-MSL-11-014C3 bibr-MSL-11-014C2 Morrison AI (bibr-MSL-11-014C16) 1972; 48 bibr-MSL-11-014C1 Nicklas JA (bibr-MSL-11-014C11) 2003; 48 bibr-MSL-11-014C14 bibr-MSL-11-014C13 bibr-MSL-11-014C12 bibr-MSL-11-014C15 |
References_xml | – volume: 14 start-page: 177 year: 1969 end-page: 83 article-title: Sperm survival and prostatic acid phosphatase activity in victims of sexual assault publication-title: J Forensic Sci contributor: fullname: Rupp – volume: 50 start-page: 1116 year: 2005 end-page: 8 article-title: Analysis of non-suspected samples lacking visually identifiable sperm using a Y-STR 10-plex publication-title: J Forensic Sci contributor: fullname: Staub – volume: 125 start-page: 212 year: 2002 end-page: 6 article-title: Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa publication-title: Forensic Sci Int contributor: fullname: Lorin de la Grandmaison – volume: 32 start-page: 678 year: 1987 end-page: 83 article-title: Persistence of vaginal spermatazoa as assessed by routine cervicovaginal (Pap) smears publication-title: J Forensic Sci contributor: fullname: Randall – volume: 3 start-page: 45 year: 1974 end-page: 55 article-title: The persistence of seminal constituents in the human vagina publication-title: Forensic Sci contributor: fullname: Wilson – volume: 136 start-page: 58 year: 2003 end-page: 72 article-title: Novel Y-STR typing strategies reveal the genetic profile of the semen donor in extended interval post-coital cervicovaginal samples publication-title: Forensic Sci Int contributor: fullname: Ballantyne – volume: 39 start-page: 1347 year: 1994 end-page: 55 article-title: Identification of sperm and non-sperm male cells in cericovaginal smears using fluorescence hybridization: applications in alleged sexual assault cases publication-title: J Forensic Sci contributor: fullname: Hayworth – volume: 48 start-page: 936 year: 2003 end-page: 44 article-title: Development of an Alu-based, realtime PCR method for quantitation of human DNA in forensic samples publication-title: J Forensic Sci contributor: fullname: Buel – volume: 48 start-page: 141 year: 1972 end-page: 3 article-title: Persistence of spermatozoa in the vagina and cervix publication-title: Br J Vener Dis contributor: fullname: Morrison – volume: 240 start-page: 1875 year: 1978 end-page: 7 article-title: Persistence of spermatozoa in the lower genital tracts of women publication-title: JAMA contributor: fullname: Silverman – volume: 53 start-page: 342 year: 2008 end-page: 8 article-title: Y-STR profiling in extended interval (>3 days) postcoital cervicovaginal samples publication-title: J Forensic Sci contributor: fullname: Ballantyne – volume: 10 start-page: 506 year: 1991 end-page: 13 article-title: Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material publication-title: BioTechniques contributor: fullname: Higuchi – volume: 1 start-page: 169 year: 1950 end-page: 75 article-title: Sperm survival at estimated ovulation time: prognostic significance publication-title: Fertil Steril contributor: fullname: Cohen – volume: 19 start-page: 561 year: 1974 end-page: 4 article-title: Sperm's morphologic survival after 16 days in the vagina of a dead body publication-title: J Forensic Sci contributor: fullname: Wilson – volume: 52 start-page: 800 year: 2007 end-page: 5 article-title: Expedited, chemically enhanced sperm cell recovery from cotton swabs for rape kit analysis publication-title: J Forensic Sci contributor: fullname: Landers – ident: bibr-MSL-11-014C2 doi: 10.1016/S0379-0738(03)00258-5 – ident: bibr-MSL-11-014C5 doi: 10.1016/0300-9432(74)90006-5 – ident: bibr-MSL-11-014C3 doi: 10.1111/j.1556-4029.2008.00672.x – ident: bibr-MSL-11-014C12 doi: 10.1520/JFS2005052 – ident: bibr-MSL-11-014C9 doi: 10.1111/j.1556-4029.2007.00453.x – ident: bibr-MSL-11-014C14 doi: 10.1001/jama.1978.03290170057027 – ident: bibr-MSL-11-014C6 doi: 10.1520/JFS12372J – volume: 48 start-page: 141 year: 1972 ident: bibr-MSL-11-014C16 publication-title: Br J Vener Dis contributor: fullname: Morrison AI – ident: bibr-MSL-11-014C1 doi: 10.1016/S0379-0738(01)00650-8 – ident: bibr-MSL-11-014C13 – volume: 48 start-page: 936 year: 2003 ident: bibr-MSL-11-014C11 publication-title: J Forensic Sci contributor: fullname: Nicklas JA – ident: bibr-MSL-11-014C7 doi: 10.1520/JFS10211J – ident: bibr-MSL-11-014C15 doi: 10.1016/S0015-0282(16)30124-8 – ident: bibr-MSL-11-014C4 doi: 10.1520/JFS13723J – volume: 14 start-page: 177 year: 1969 ident: bibr-MSL-11-014C8 publication-title: J Forensic Sci contributor: fullname: Rupp JC – volume: 10 start-page: 506 year: 1991 ident: bibr-MSL-11-014C10 publication-title: BioTechniques contributor: fullname: Walsh PS |
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Snippet | Y-STR profiles using the Promega PowerPlex® Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from... Y-STR profiles using the Promega PowerPlex(®) Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from... Y-STR profiles using the Promega PowerPlex ® Y system were attempted on multiple vaginal swabs collected at four, six and eight days after intercourse from... |
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SubjectTerms | Chromosomes, Human, Y Coitus DNA Fingerprinting - methods Female Genotype Haplotypes Humans Male Microsatellite Repeats Polymerase Chain Reaction Semen - chemistry Time Factors Vagina - chemistry |
Title | The utility of Y-STR profiling in four-, six- and eight-day postcoital vaginal swabs |
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