Temperature changes in lipid and protein structure measured by fourier transform infrared spectrophotometry in intact pollen grains

Fourier Transform infrared (FTIR) spectroscopy was used to monitor structural changes in lipids and proteins in four species of pollen grains at different temperatures. Spectral data were used to determine membrane phase transition temperature ( Tm) in pollen at two hydration levels. Imbibition was...

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Published inPlant science (Limerick) Vol. 78; no. 1; pp. 1 - 9
Main Authors Sowa, Sharon, Connor, Kristina F., Towill, Leigh E.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 1991
Elsevier Science
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
imbibitional damage
infrared
membrane transition
Plant physiology and development
pollen
Sexual reproduction
Vegetative and sexual reproduction, floral biology, fructification
imbibitional damage
infrared
pollen
membrane transition
Monocotyledones
Thermal factor
Hydration
Molecular structure
Lipids
Proteins
Infrared spectrometry
Typhaceae
Juglandaceae
Carya illinoensis
Pinus ponderosa
Dicotyledones
Angiospermae
Gymnospermae
Coniferales
Spermatophyta
Pollen
Typha latifolia
Picea pungens
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Abstract Fourier Transform infrared (FTIR) spectroscopy was used to monitor structural changes in lipids and proteins in four species of pollen grains at different temperatures. Spectral data were used to determine membrane phase transition temperature ( Tm) in pollen at two hydration levels. Imbibition was conducted at temperatures above and below the determined Tm values to correlate damage to the presence of gel phase. For three species of pollen, hydration overcame imbibitional damage at low temperature. However, dry pecan pollen, which had a high lipid content, was injured during imbibition at temperatures above and below the Tm. Changes in protein structure were not related to imbibitional damage. FTIR spectroscopy is a useful tool in examining biochemical properties of intact pollen grains and has potential for evaluating cryopreservation protocols.
AbstractList Fourier Transform infrared (FTIR) spectroscopy was used to monitor structural changes in lipids and proteins in four species of pollen grains at different temperatures. Spectral data were used to determine membrane phase transition temperature ( Tm) in pollen at two hydration levels. Imbibition was conducted at temperatures above and below the determined Tm values to correlate damage to the presence of gel phase. For three species of pollen, hydration overcame imbibitional damage at low temperature. However, dry pecan pollen, which had a high lipid content, was injured during imbibition at temperatures above and below the Tm. Changes in protein structure were not related to imbibitional damage. FTIR spectroscopy is a useful tool in examining biochemical properties of intact pollen grains and has potential for evaluating cryopreservation protocols.
Author Towill, Leigh E.
Sowa, Sharon
Connor, Kristina F.
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  fullname: Connor, Kristina F.
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  givenname: Leigh E.
  surname: Towill
  fullname: Towill, Leigh E.
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Cites_doi 10.2307/1931961
10.1021/bi00337a016
10.1016/S0176-1617(88)80197-4
10.1016/0141-0229(86)90043-8
10.1111/j.1432-1033.1988.tb14455.x
10.1007/BF00468375
10.1104/pp.95.2.610
10.2307/2444223
10.1016/0005-2736(89)90198-3
10.1126/science.6849129
10.1016/0011-2240(89)90035-7
10.1016/0304-4157(84)90017-0
10.2307/2439772
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Issue 1
Keywords imbibitional damage
infrared
pollen
membrane transition
Monocotyledones
Thermal factor
Hydration
Molecular structure
Lipids
Proteins
Infrared spectrometry
Typhaceae
Juglandaceae
Carya illinoensis
Pinus ponderosa
Dicotyledones
Angiospermae
Gymnospermae
Coniferales
Spermatophyta
Pollen
Typha latifolia
Picea pungens
Language English
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PublicationTitle Plant science (Limerick)
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Publisher Elsevier Ireland Ltd
Elsevier Science
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SubjectTerms Biological and medical sciences
Fundamental and applied biological sciences. Psychology
imbibitional damage
infrared
membrane transition
Plant physiology and development
pollen
Sexual reproduction
Vegetative and sexual reproduction, floral biology, fructification
Title Temperature changes in lipid and protein structure measured by fourier transform infrared spectrophotometry in intact pollen grains
URI https://dx.doi.org/10.1016/0168-9452(91)90155-2
Volume 78
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