Triploidy induced by heat shock and hydrostatic pressure in landlocked Atlantic salmon ( Salmo salar L.)

• Published in: Aquaculture Vol. 36; no. 4; pp. 359 - 367
• Main Authors: Benfey, Tillmann J., Sutterlin, Arnold M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.02.1984; Elsevier Science
• Subjects: Animals; Biological and medical sciences; Cytogenetics; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Freshwater environment; Vertebrata; Temperature; Induction; Pisces; Triploidy; Osmotic pressure
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/0044-8486(84)90328-4

Heat shocks of 5 min at 32°C or hydrostatic pressure shocks of 3 or 6 min at 7.0 × 10 4 kPa (10 150 p.s.i.), when completed within 20 min of fertilization at 10°C, were found to induce 100% triploidy with 70–90% survival (relative to controls) in landlocked Atlantic salmon ( Salmo salar L.). The identical heat shock yielded substantially lower numbers of triploids when applied 25 to 45 min after fertilization. Pressure shocks of longer duration (9 to 15 min at 7.0 × 10 4 kPa) or higher magnitude (6 min at 7.9 × 10 4 to 10.5 × 10 4 kPa) resulted in 100% mortality prior to hatching. Possible applications of these techniques to salmonid aquaculture are discussed.