Evaluation of a high‐content screening fluorescence‐based assay analyzing the pharmacological modulation of lipid homeostasis in human macrophages

• Published in: Cytometry. Part A Vol. 69A; no. 3; pp. 200 - 202
• Main Authors: Werner, Tobias, Liebisch, Gerhard, Grandl, Margot, Schmitz, Gerd
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.03.2006
• Subjects: Cell Membrane - drug effects; Cell Membrane - metabolism; Cholesterol - pharmacology; fluorescence; Fluorescent Dyes - chemistry; high‐content screening; Homeostasis - drug effects; Humans; Image Cytometry - methods; lipid homeostasis; Lipid Metabolism - drug effects; Lipoproteins, HDL - pharmacology; Lipoproteins, HDL3; Lipoproteins, LDL - chemistry; Lipoproteins, LDL - pharmacology; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Membrane Microdomains - drug effects; Membrane Microdomains - metabolism; Phosphatidylethanolamines - chemistry; Reproducibility of Results; Rhodamines - chemistry

Background For understanding cholesterol and phospholipid efflux pathways there is a need for cellular fluorescence‐based high‐content screens (HCS) to investigated the cholesterol and phospholipid content in human macrophages. Methods Making use of fluorescence imaging based on HCS we have develope...