Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats

• Published in: Mutagenesis Vol. 30; no. 3; pp. 315 - 324
• Main Authors: Revollo, Javier, Pearce, Mason G., Petibone, Dayton M., Mittelstaedt, Roberta A., Dobrovolsky, Vasily N.
• Format: Journal Article
• Language: English
• Published: England 01.05.2015
• Subjects: Animals; Antigens, CD - genetics; Antigens, CD - metabolism; Base Sequence; CD48 Antigen; Cells, Cultured; DNA Mutational Analysis; Ethylnitrosourea - pharmacology; Flow Cytometry; High-Throughput Nucleotide Sequencing; Immunomagnetic Separation; Male; Membrane Proteins - genetics; Mutagenesis; Mutagenicity Tests; Mutagens - pharmacology; Mutation; Phenotype; Rats, Inbred F344; T-Lymphocytes - metabolism

The Pig-a assay is used for monitoring somatic cell mutation in laboratory animals and humans. The assay detects haematopoietic cells deficient in glycosylphosphatidylinositol (GPI)-anchored protein surface markers using flow cytometry. However, given that synthesis of the protein markers (and the e...