Expression of the BnmNAP subfamily of napin genes coincides with the induction of Brassica microspore embryogenesis
Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene...
Saved in:
Published in | Plant molecular biology Vol. 26; no. 6; p. 1711 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
01.12.1994
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene expression. Changes in the plant growth or microspore culture conditions were not by themselves sufficient to induce napin gene expression. Specific members of the napin multigene family were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of napin clones represented three members (BnmNAP2, BnmNAP3 and BnmNAP4) that, along with a previously isolated napin genomic clone (BngNAP1), constitute the highly conserved BnmNAP subfamily of napin genes. Both RNA gel blot analysis, using a subfamily-specific probe, and histochemical analysis of transgenic plants expressing a BngNAP1 promoter-beta-glucuronidase gene fusion demonstrated that the BnmNAP subfamily is expressed in embryogenic microspores as well as during subsequent stages of microsporic embryo development. |
---|---|
AbstractList | Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene expression. Changes in the plant growth or microspore culture conditions were not by themselves sufficient to induce napin gene expression. Specific members of the napin multigene family were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of napin clones represented three members (BnmNAP2, BnmNAP3 and BnmNAP4) that, along with a previously isolated napin genomic clone (BngNAP1), constitute the highly conserved BnmNAP subfamily of napin genes. Both RNA gel blot analysis, using a subfamily-specific probe, and histochemical analysis of transgenic plants expressing a BngNAP1 promoter-beta-glucuronidase gene fusion demonstrated that the BnmNAP subfamily is expressed in embryogenic microspores as well as during subsequent stages of microsporic embryo development. |
Author | Huang, B Miki, B.L Gines, M.J Boutilier, K.A. (Ottawa Univ., Ottawa, Ont. (Canada). Dept. of Biology) DeMoor, J.M Baszczynski, C.L Iyer, V.N |
Author_xml | – sequence: 1 fullname: Boutilier, K.A. (Ottawa Univ., Ottawa, Ont. (Canada). Dept. of Biology) – sequence: 2 fullname: Gines, M.J – sequence: 3 fullname: DeMoor, J.M – sequence: 4 fullname: Huang, B – sequence: 5 fullname: Baszczynski, C.L – sequence: 6 fullname: Iyer, V.N – sequence: 7 fullname: Miki, B.L |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7858212$$D View this record in MEDLINE/PubMed |
BookMark | eNotkM1OwzAQhC1UVNrChSMSkl8gsHbixDm2VQtIVeEA58h_aY0aO7JTQd6e0Pa0o52dT5qdopHzziB0T-CJABTPizUAkDLj-RWaEFakCQPKR2gCJC-SLCP0Bk1j_B6OANJ8jMYFZ5wSOkFx9dsGE6P1Dvsad3uDF67Zzj9wPMpaNPbQ_--daK3DO-NMxMpbp6we1I_t9qeIdfqougtjEcTAUwI3VgUfWx8MNo0MvT_lbbxF17U4RHN3mTP0tV59Ll-TzfvL23K-SVSa0i7RhWZSGAWE6bIuWZlqxlMtZUklMxwYGAOMa5AcDJR5kXFecK0zrVPCFaUz9HjmtkfZGF21wTYi9NWl_OA_nP1a-Ersgo3VdlPmw9M40D8hU2Zf |
CitedBy_id | crossref_primary_10_1007_s11033_010_0587_z crossref_primary_10_1186_s12864_022_08907_9 crossref_primary_10_1016_S0176_1617_99_80277_6 crossref_primary_10_1371_journal_pone_0110065 crossref_primary_10_1016_j_tibtech_2015_07_005 crossref_primary_10_1023_A_1002835330799 crossref_primary_10_1007_s00425_004_1371_x crossref_primary_10_1134_S1068162014010130 crossref_primary_10_1046_j_1365_313X_2003_01714_x crossref_primary_10_1073_pnas_1813993115 crossref_primary_10_1093_jxb_eri190 crossref_primary_10_1104_pp_124_2_523 crossref_primary_10_1111_j_1399_3054_2006_00673_x crossref_primary_10_1016_j_biotechadv_2008_05_006 crossref_primary_10_1016_S1360_1385_97_89951_7 crossref_primary_10_3390_cimb45050282 crossref_primary_10_1034_j_1399_3054_2001_1110101_x crossref_primary_10_1111_j_1399_3054_2004_00391_x crossref_primary_10_1016_S0176_1617_00_80303_X crossref_primary_10_1007_s00425_009_0929_z crossref_primary_10_1023_B_JOPC_0000027849_08211_85 crossref_primary_10_1104_pp_106_092932 crossref_primary_10_1007_s11816_022_00752_2 crossref_primary_10_1371_journal_pone_0110372 crossref_primary_10_1007_BF00020480 crossref_primary_10_1007_s11756_021_00721_0 crossref_primary_10_1007_s12298_021_01052_9 crossref_primary_10_1134_S1021443709050112 crossref_primary_10_1186_s12864_019_5948_y crossref_primary_10_1002_ece3_1792 crossref_primary_10_1139_g02_032 crossref_primary_10_1007_s00299_017_2197_x crossref_primary_10_1038_srep38401 crossref_primary_10_1007_s11103_009_9541_7 crossref_primary_10_1007_s11103_006_9090_2 crossref_primary_10_1007_s11103_009_9526_6 crossref_primary_10_1186_1471_2229_10_197 crossref_primary_10_3389_fpls_2016_00485 crossref_primary_10_1080_07352689709701959 crossref_primary_10_1007_s00425_006_0388_8 crossref_primary_10_1007_s11627_008_9128_z crossref_primary_10_1007_s12298_023_01393_7 |
ContentType | Journal Article |
DBID | FBQ CGR CUY CVF ECM EIF NPM |
DOI | 10.1007/BF00019486 |
DatabaseName | AGRIS Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Biology Botany |
EISSN | 1573-5028 |
ExternalDocumentID | 7858212 NL9601680 |
Genre | Research Support, Non-U.S. Gov't Journal Article Comparative Study |
GroupedDBID | --- -4W -56 -5G -BR -EM -Y2 -~C .86 06C 06D 0R~ 0VY 123 199 1N0 1SB 2.D 203 28- 29O 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 3SX 3V. 4.4 406 408 409 40D 40E 53G 5QI 5VS 67N 67Z 6NX 78A 7X7 88A 88E 8AO 8FE 8FH 8FI 8FJ 8G5 8TC 8UJ 95- 95. 95~ 96X A8Z AAAVM AABHQ AABYN AAFGU AAGAY AAHNG AAIAL AAJKR AANXM AANZL AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABBBX ABBXA ABDZT ABECU ABELW ABFGW ABFSI ABFTV ABHLI ABHQN ABJNI ABJOX ABKAS ABKCH ABKTR ABLJU ABMNI ABMQK ABNWP ABPLI ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFS ACHSB ACHXU ACIGE ACIPQ ACKNC ACMDZ ACMLO ACNCT ACOKC ACOMO ACPRK ACTTH ACVWB ACWMK ADBBV ADHHG ADHIR ADIMF ADINQ ADKNI ADKPE ADMDM ADOAH ADOXG ADRFC ADTPH ADURQ ADYFF ADYPR ADZKW AEBTG AEEQQ AEFIE AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETLH AEVLU AEVTX AEXYK AFEXP AFGCZ AFKRA AFLOW AFNRJ AFQWF AFWTZ AFZKB AGAYW AGDGC AGGBP AGGDS AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AJZVZ AKMHD AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG AOSHJ ARMRJ ASPBG AVWKF AXYYD AZFZN AZQEC B-. BA0 BBNVY BBWZM BDATZ BENPR BGNMA BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CSCUP DDRTE DL5 DNIVK DPUIP DU5 DWQXO EBD EBLON EBS ECGQY EIOEI EJD EMOBN EN4 EPAXT ESBYG ESTFP F5P FA8 FBQ FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNUQQ GNWQR GQ6 GQ7 GQ8 GUQSH GXS HCIFZ HF~ HG5 HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IAG IAO IHE IJ- IKXTQ ITM IWAJR IXC IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KOW KPH LAK LK8 LLZTM M0L M1P M2O M4Y M7P MA- MQGED MVM N2Q NB0 NDZJH NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM OVD P0- P19 PF- PQQKQ PROAC PSQYO PT4 PT5 Q2X QOK QOR QOS R4E R89 R9I RHV RNI ROL RPX RRX RSV RZC RZE RZK S16 S1Z S26 S27 S28 S3A S3B SAP SBL SBY SCLPG SDH SDM SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZN T13 T16 TEORI TSG TSK TSV TUC U2A U9L UG4 UKHRP UNUBA UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WH7 WJK WK6 WK8 Y6R YLTOR YZZ Z45 Z7U Z7V Z7W Z7Y Z83 Z8O Z8P Z8Q Z8S Z8W ZMTXR ZOVNA ~EX ~KM AACDK AAEOY AAHBH AAJBT AAQLM AASML ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGQEE AGRTI AIGIU ALIPV CGR CUY CVF ECM EIF H13 ITC NPM |
ID | FETCH-LOGICAL-c332t-d7d5baec015d9f9593d583dbb92b5e8050ee058d0b80e096748878dd4dd318c22 |
ISSN | 0167-4412 |
IngestDate | Sat Sep 28 08:36:24 EDT 2024 Wed Dec 27 19:15:22 EST 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c332t-d7d5baec015d9f9593d583dbb92b5e8050ee058d0b80e096748878dd4dd318c22 |
Notes | 9601680 F30 |
PMID | 7858212 |
ParticipantIDs | pubmed_primary_7858212 fao_agris_NL9601680 |
PublicationCentury | 1900 |
PublicationDate | 1994-12-01 |
PublicationDateYYYYMMDD | 1994-12-01 |
PublicationDate_xml | – month: 12 year: 1994 text: 1994-12-01 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | Netherlands |
PublicationPlace_xml | – name: Netherlands |
PublicationTitle | Plant molecular biology |
PublicationTitleAlternate | Plant Mol Biol |
PublicationYear | 1994 |
SSID | ssj0010036 |
Score | 1.7281455 |
Snippet | Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress... |
SourceID | pubmed fao |
SourceType | Index Database Publisher |
StartPage | 1711 |
SubjectTerms | 2S Albumins, Plant Base Sequence Brassica - embryology Brassica - genetics BRASSICA NAPUS Cells, Cultured DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE DNA, Complementary - genetics EMBRYONIC DEVELOPMENT EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Gene Library Genes, Plant - genetics Genetic Vectors Germ Cells Glucuronidase - biosynthesis Glucuronidase - genetics Haploidy Molecular Sequence Data Multigene Family - genetics Oligonucleotide Probes Plant Proteins - biosynthesis Plant Proteins - genetics Pollen - growth & development PROTEINAS DE RESERVA PROTEINE DE RESERVE Recombinant Fusion Proteins - biosynthesis Sequence Homology, Nucleic Acid STORAGE PROTEINS Transformation, Genetic |
Title | Expression of the BnmNAP subfamily of napin genes coincides with the induction of Brassica microspore embryogenesis |
URI | https://www.ncbi.nlm.nih.gov/pubmed/7858212 |
Volume | 26 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELZ2gUq9IFioWh6VD9yiIOdVe49daKkqugKplXpb-RW0hySr7q7E8gP43czY3iRbQDwuUeRHlHi-2DPj8TeEvCmFUplMZZzlksd5am2sjGZxavnJOJG6lI6n4Gp6cnGTX94Wt4PB917U0nql3upvvzxX8j9ShTKQK56S_QfJtg-FArgH-cIVJAzXv5Lx2dcQxlq3W_11NT39FC3XKnguME5DLuY1pkq2y0g36Fs3Nhxqwy5glHsGWWw7uQNlGuQWVRiohyavjWyl7jaN6z9f9pVZTHi0iqptgt0oEDq1Fn4Dnw4qro_a6JymH-a13573ju5uY-q9vWq89-Cy56RdB4_2pHNQOKbhLtgj-CyRWj1PdiZdf0w-gKs_gybcT74_Te1sG7COWmnu-bN7Ml5UTshc4Mnf9I-V90i2Q82QDLnAeXKKPp9tHgzmEk22n7FLchveZp_shWeAklLK5p6V4rSV6yfkcTAz6KnHzFMysPWI7PnEo5sReTRpwCjYPCPLDkK0KSnggXoI0RZCWO4gRB0EaAshihByXVoIYdsthGgHIboDoefk5vzs-t1FHPJwxDrL0lVsuCmUtBo0RzMukcnaFCIzSo1TVVjBCmYtK4RhSjALJjGHRYELY3JjYMXQaXpAHtRNbQ8J1To1ZWkSBW1znhulsyIxhWZc57y0yREZweDN5BdY4WbTj2MkCxLsiBz4wZwtPAXLLIz1i99VvCT7HRRfkYcl_PH2NaiPK3VMhueTz8dOxj8A0eJwRA |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Expression+of+the+BnmNAP+subfamily+of+napin+genes+coincides+with+the+induction+of+Brassica+microspore+embryogenesis&rft.jtitle=Plant+molecular+biology&rft.au=Boutilier%2C+K+A&rft.au=Gin%C3%A9s%2C+M+J&rft.au=DeMoor%2C+J+M&rft.au=Huang%2C+B&rft.date=1994-12-01&rft.issn=0167-4412&rft.volume=26&rft.issue=6&rft.spage=1711&rft_id=info:doi/10.1007%2FBF00019486&rft_id=info%3Apmid%2F7858212&rft_id=info%3Apmid%2F7858212&rft.externalDocID=7858212 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0167-4412&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0167-4412&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0167-4412&client=summon |