Genetic engineering and overexpression of ribosomal L12 protein genes from three different archaebacteria in E coli

• Published in: Biochimie Vol. 73; no. 6; pp. 647 - 655
• Main Authors: Köpke, A.K.E., Hannemann, F., Boeckh, T.
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01.06.1991; Elsevier
• Subjects: Amino Acids - analysis; Archaea - genetics; Base Sequence; Biological and medical sciences; Biotechnology; Chromatography, Ion Exchange; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; gene modifications; Genetic Engineering; Genetic technics; Genetic Vectors; Methods. Procedures. Technologies; Miscellaneous; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligonucleotides - chemical synthesis; overexpression; protein purification; ribosomal proteins; Ribosomal Proteins - biosynthesis; Ribosomal Proteins - genetics; Ribosomal Proteins - isolation & purification; Synthetic digonucleotides and genes. Sequencing; ribosomal proteins; protein purification; gene modifications; overexpression; DTE; DMF; Purification; Archaeobacteria; Escherichia coli; HPLC chromatography; Gene expression; Halobacterium halobium; Ribosomal protein; Biological activity; Ion exchange chromatography; Gene; Bacteria; Two dimensional electrophoresis; Mutation; Halobacteriaceae; Enterobacteriaceae

Genes coding for ribosomal protein L12 from Methanococcus vannielii (Mva), Halobacterium halobium (Hha) and Sulfolobus solfataricus (Sso) have been subcloned in the polylinker region of pUC19. An efficient Shine-Dalgarno sequence has been attached to the 5′ end of the genes, and two ochre stop codon...