Impaired in-vitro proliferation of hemopoietic precursors in HIV-1-infected subjects

• Published in: Leukemia research Vol. 13; no. 7; pp. 573 - 581
• Main Authors: Lunardi-Iskandar, Y., Georgoulias, V., Bertoli, A.M., Augery-Bourget, Y., Ammar, A., Vittecoq, D., Rosenbaum, W., Meyer, P., Jasmin, C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1989; Elsevier Science
• Subjects: Acquired Immunodeficiency Syndrome - blood; Acquired Immunodeficiency Syndrome - pathology; AIDS; Antigen-Presenting Cells - physiology; Antigen-Presenting Cells - radiation effects; Biological and medical sciences; Bone Marrow - pathology; Cell Adhesion; Cell Division - drug effects; Cell Separation; Colony-Forming Units Assay; Colony-Stimulating Factors - pharmacology; Female; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances - pharmacology; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - pathology; hemopoietic precursors; HIV; Humans; Immunodeficiencies; Immunodeficiencies. Immunoglobulinopathies; Immunopathology; Macrophages - pathology; Male; Medical sciences; Monocytes - pathology; Recombinant Proteins - pharmacology; T-Lymphocytes - physiology; T-Lymphocytes - radiation effects; HIV, human immunodeficiency virus; ARC, AIDS-related complex; AIDS; BMMCA −, non-adherent bone marrow mononuclear cells; AIDS, acquired immunodeficiency syndrome; rIL3, recombinant Interleukin 3; A + p, adherent cells from HIV-patients; rGM-CSF, recombinant granulocyte/macrophage colony-stimulating factor; CD2 −, CD3 − cells, T cell-depleted cells; HIV; A + n, normal adherent cells; LAS, persistent lymphadenopathy syndrome; E + n, normal T cells; PHA-LCM, 7-day medium conditioned by PHA-activated normal T cells; Epo, erythropoietin; hemopoietic precursors; Human; Cell culture; Immunopathology; HIV-1 virus; Retroviridae; Hematopoietic cell; Lymphadenopathy syndrome; Hemopathy; Infection; Virus; Clonogenic assay; CFU-BL»-Cell; Pluripotent cell; Viral disease; CFU-GM-Cell; Human immunodeficiency virus; Growth factor

Patients with acquired immunodeficiency syndrome (AIDS) and persistent lymphadenopathy syndrome (LAS) display significant hematological abnormalities of one or more cell lineages. In order to understand the pathophysiologic mechanisms leading to these abnormalities we studied the proliferation capac...