Impaired in-vitro proliferation of hemopoietic precursors in HIV-1-infected subjects

• Published in: Leukemia research Vol. 13; no. 7; pp. 573 - 581
• Main Authors: Lunardi-Iskandar, Y., Georgoulias, V., Bertoli, A.M., Augery-Bourget, Y., Ammar, A., Vittecoq, D., Rosenbaum, W., Meyer, P., Jasmin, C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1989; Elsevier Science
• Subjects: Acquired Immunodeficiency Syndrome - blood; Acquired Immunodeficiency Syndrome - pathology; AIDS; Antigen-Presenting Cells - physiology; Antigen-Presenting Cells - radiation effects; Biological and medical sciences; Bone Marrow - pathology; Cell Adhesion; Cell Division - drug effects; Cell Separation; Colony-Forming Units Assay; Colony-Stimulating Factors - pharmacology; Female; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances - pharmacology; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - pathology; hemopoietic precursors; HIV; Humans; Immunodeficiencies; Immunodeficiencies. Immunoglobulinopathies; Immunopathology; Macrophages - pathology; Male; Medical sciences; Monocytes - pathology; Recombinant Proteins - pharmacology; T-Lymphocytes - physiology; T-Lymphocytes - radiation effects; HIV, human immunodeficiency virus; ARC, AIDS-related complex; AIDS; BMMCA −, non-adherent bone marrow mononuclear cells; AIDS, acquired immunodeficiency syndrome; rIL3, recombinant Interleukin 3; A + p, adherent cells from HIV-patients; rGM-CSF, recombinant granulocyte/macrophage colony-stimulating factor; CD2 −, CD3 − cells, T cell-depleted cells; HIV; A + n, normal adherent cells; LAS, persistent lymphadenopathy syndrome; E + n, normal T cells; PHA-LCM, 7-day medium conditioned by PHA-activated normal T cells; Epo, erythropoietin; hemopoietic precursors; Human; Cell culture; Immunopathology; HIV-1 virus; Retroviridae; Hematopoietic cell; Lymphadenopathy syndrome; Hemopathy; Infection; Virus; Clonogenic assay; CFU-BL»-Cell; Pluripotent cell; Viral disease; CFU-GM-Cell; Human immunodeficiency virus; Growth factor
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/0145-2126(89)90124-0

Patients with acquired immunodeficiency syndrome (AIDS) and persistent lymphadenopathy syndrome (LAS) display significant hematological abnormalities of one or more cell lineages. In order to understand the pathophysiologic mechanisms leading to these abnormalities we studied the proliferation capacity of pluripotent and committed hemopoietic precursors using in-vitro colony assays. Anemia, leukopenia and thrombopenia were relatively frequent findings in HIV-infected subjects irrespectively of the patients' clinical status. The colony growth capacity of AIDS patients' GM-CFU and BFU-E was significantly decreased whereas no GEMM-CFU colonies could be obtained. There was no correlation between the number of BFU-E and GM-CFU colony number and the hemoglobin or the absolute number of polynuclear cells, respectively. The plating efficiency of both committed and pluripotent hematopoietic precursors from HIV infected patients could not be enhanced when additional exogenous recombinant GM-CSF, human interleukin 3 or erythropoietin were added in contrast to normal patients' cells. In addition, the impaired colony growth of these precursors could not be restored after adherent or T-cell depletion or the addition of normal allogenic irradiated adherent or/and T cells. Since this colony growth abnormality was also detected in HIV seropositive asymptomatic subjects our findings strongly suggest that the in-vitro growth of hematopoietic precursors is affected early after HIV-1 infection.