Immunosensing with photo-immobilized immunoreagents on planar optical wave guides

• Published in: Biosensors & bioelectronics Vol. 10; no. 3; pp. 317 - 328
• Main Authors: Gao, Hui, Sänger, Michael, Luginbühl, Reto, Sigrist, Hans
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 1995; Elsevier Science
• Subjects: antibody immobilization; Antigen-Antibody Reactions; Biological and medical sciences; Biosensing Techniques; Biosensors; Biotechnology; enzyme-linked immunoassay; Fundamental and applied biological sciences. Psychology; Immobilization of enzymes and other molecules; Immobilization techniques; immunochemical sensor; Immunoglobulins - analysis; integrated optics; Methods. Procedures. Technologies; photo-immobilization; Photochemistry; Space life sciences; titanium oxide/silicon oxide; Various methods and equipments; immunochemical sensor; titanium oxide/silicon oxide; enzyme-linked immunoassay; integrated optics; antibody immobilization; photo-immobilization; Bovine; Immobilization; Serum albumin; Monoclonal antibody; Photochemical reaction; Immunosensor; Surface; Vertebrata; Mammalia; Biosensor; Covalent bond; F(ab')2-Fragment; Optical waveguide; Artiodactyla; Titanium oxide; Ungulata

Immunocomplexation at wave guiding TiO 2/SiO 2 surfaces was investigated using an integrated optical grating coupler. For extended application of this label-free monitoring system, F(ab′) 2 fragments of monoclonal antibodies were photo-immobilized by photolinker polymer-mediated procedures that do n...