Forced overexpression of either of the two common human Foxp3 isoforms can induce regulatory T cells from CD4⁺CD25⁻ cells

• Published in: European journal of immunology Vol. 38; no. 5; pp. 1381 - 1390
• Main Authors: Aarts-Riemens, Tineke, Emmelot, Maarten E, Verdonck, Leo F, Mutis, Tuna
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.05.2008; WILEY‐VCH Verlag
• Subjects: Antibodies - pharmacology; Antigens, CD - analysis; CD3 Complex - immunology; CD4-Positive T-Lymphocytes - cytology; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; Cell Differentiation - immunology; Cell Proliferation - drug effects; Coculture Techniques; Cytokines - metabolism; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Foxp3 isoforms; Gene Expression; Human; Humans; Immune Tolerance - immunology; Immunophenotyping; Interferon-gamma - metabolism; Interleukin-2 Receptor alpha Subunit - analysis; Interleukins - metabolism; Leukocytes, Mononuclear - metabolism; Protein Isoforms - genetics; Protein Isoforms - metabolism; Regulatory T cells; Retroviral transduction; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism; T-Lymphocytes, Regulatory - cytology; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - metabolism; Transcription factors; Transfection; Tumor Necrosis Factor-alpha - metabolism

The forkhead/winged helix transcription factor (Foxp3) is expressed as two different isoforms in humans: the full-length isoform (Foxp3FL) and an alternative-splicing product lacking the exon 2 (Foxp3ΔE2). We here studied the cellular distribution of Foxp3 isoforms by quantitative PCR and evaluated...