Multimodal 3-D/2-D human islet and duct imaging in exocrine and endocrine lesion environment: associated pancreas tissue remodeling

• Published in: American journal of physiology: endocrinology and metabolism Vol. 323; no. 4; pp. E354 - E365
• Main Authors: Chung, Mei-Hsin, Chien, Hung-Jen, Peng, Shih-Jung, Chou, Ya-Hsien, Chiang, Tsai-Chen, Chang, Hsiu-Pi, Lee, Chih-Yuan, Chen, Chien-Chia, Jeng, Yung-Ming, Tien, Yu-Wen, Tang, Shiue-Cheng
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.10.2022
• Subjects: Adenocarcinoma; Agglomeration; Cadaver; Cadavers; Cancer; Carcinoma in Situ - pathology; Carcinoma, Pancreatic Ductal - diagnostic imaging; Carcinoma, Pancreatic Ductal - pathology; Histology; Humans; Lesions; Margins of Excision; Medical imaging; Microenvironments; Neoplasia; Neoplasms; Neuroendocrine tumors; Pancreas; Pancreas - metabolism; Pancreatic cancer; Pancreatic Neoplasms; Pancreatic Neoplasms - diagnostic imaging; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Stereomicroscopy; Surveys; Tumor Microenvironment; islet cell microadenoma; islet aggregation; pancreatic intraepithelial neoplasia; pancreatic neuroendocrine tumor; pancreatic ductal adenocarcinoma

We develop a new multimodal 3-D/2-D imaging approach (matched stereomicroscopic, fluorescence, and H&E signals) to examine human duct-islet association in the PDAC surgical margin and cadaveric donor pancreas. In both conditions, peri-PanIN islet aggregation with duct-islet cell clusters was ide...