Apoptosis in the rat mammary gland and ventral prostate: detection of cell death-associated genes using a coincident-expression cloning approach
Apoptosis plays a striking role in the hormone-dependent involution of the mammary gland, but it has proved difficult to distinguish between the 'cell death' associated genes and the 'tissue remodelling' genes which are expressed concurrently. To identify cell death-associated ge...
Saved in:
Published in | Cell death and differentiation Vol. 4; no. 2; pp. 114 - 124 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.02.1997
|
Online Access | Get full text |
Cover
Loading…
Abstract | Apoptosis plays a striking role in the hormone-dependent involution of the mammary gland, but it has proved difficult to distinguish between the 'cell death' associated genes and the 'tissue remodelling' genes which are expressed concurrently. To identify cell death-associated genes, we have established a 'coincidence analysis' based on the previously described 'RNA differential display' method of Liang and Pardee (1992). Coincidence analysis allows the detection of genes expressed during related processes in different organs and was employed here to identify transcripts in which expression patterns are seen to be associated with apoptosis during involution of both rat mammary- and the ventral prostate glands. That the coincidence analysis is a promising approach can be seen from the fact that while widely accepted apoptosis markers such as transglutaminase (Fesus et al, 1987; Strange et al, 1992) and sulfated glycoprotein-2 (Buttyan et al, 1989; Strange et al, 1992; Guenette et al 1994) exhibited similar expression in both regressing tissues, transcription of tissue remodelling enzymes was minimal in the involuting prostate. We describe here the characteristics of five clones isolated which show coincident expression during programmed cell death in mammary and prostate tissues. Partial sequence analysis revealed for three clones high homologies with previously described genes; the putative rat homolog of the growth arrest gene gas-1 (Schneider et al, 1988; Del Sal et al, 1992), an homolog of the mouse 'Integrin Associated Protein' (IAP) (Brown et al, 1990; Lindberg et al, 1993) and the sequence encoding for the 'Allograft Inflammatory Factor' AIF-1 (Autieri et al, 1995; Utans et al, 1995). One clone displayed homology with an expressed human sequence tag and one clone unrelated to any known DNA sequence was isolated. The expression of these genes in involuting rat mammary and ventral prostate, was correlated with that in other organs and in situ hybridization was applied to establish that the secretory epithelial cells which undergo programmed cell death are the site of elevated expression during the course of involution. Furthermore, we conclude that the coincidence analysis approach described here could be easily applied to facilitate the characterization of gene expression i.e. for the detection and comparison of hormonally regulated genes in different organs. |
---|---|
AbstractList | Apoptosis plays a striking role in the hormone-dependent involution of the mammary gland, but it has proved difficult to distinguish between the 'cell death' associated genes and the 'tissue remodelling' genes which are expressed concurrently. To identify cell death-associated genes, we have established a 'coincidence analysis' based on the previously described 'RNA differential display' method of Liang and Pardee (1992). Coincidence analysis allows the detection of genes expressed during related processes in different organs and was employed here to identify transcripts in which expression patterns are seen to be associated with apoptosis during involution of both rat mammary- and the ventral prostate glands. That the coincidence analysis is a promising approach can be seen from the fact that while widely accepted apoptosis markers such as transglutaminase (Fesus et al, 1987; Strange et al, 1992) and sulfated glycoprotein-2 (Buttyan et al, 1989; Strange et al, 1992; Guenette et al 1994) exhibited similar expression in both regressing tissues, transcription of tissue remodelling enzymes was minimal in the involuting prostate. We describe here the characteristics of five clones isolated which show coincident expression during programmed cell death in mammary and prostate tissues. Partial sequence analysis revealed for three clones high homologies with previously described genes; the putative rat homolog of the growth arrest gene gas-1 (Schneider et al, 1988; Del Sal et al, 1992), an homolog of the mouse 'Integrin Associated Protein' (IAP) (Brown et al, 1990; Lindberg et al, 1993) and the sequence encoding for the 'Allograft Inflammatory Factor' AIF-1 (Autieri et al, 1995; Utans et al, 1995). One clone displayed homology with an expressed human sequence tag and one clone unrelated to any known DNA sequence was isolated. The expression of these genes in involuting rat mammary and ventral prostate, was correlated with that in other organs and in situ hybridization was applied to establish that the secretory epithelial cells which undergo programmed cell death are the site of elevated expression during the course of involution. Furthermore, we conclude that the coincidence analysis approach described here could be easily applied to facilitate the characterization of gene expression i.e. for the detection and comparison of hormonally regulated genes in different organs. |
Author | Saurer, S Bielke, W Friis, R R Feng, Z Ke, G Buhrer, S |
Author_xml | – sequence: 1 givenname: W surname: Bielke fullname: Bielke, W organization: Laboratory for Clinical and Experimental Research, University of Berne, Tiefenaustrasse 120, CH-3004 Bern, Switzerland – sequence: 2 givenname: G surname: Ke fullname: Ke, G – sequence: 3 givenname: Z surname: Feng fullname: Feng, Z – sequence: 4 givenname: S surname: Buhrer fullname: Buhrer, S – sequence: 5 givenname: S surname: Saurer fullname: Saurer, S – sequence: 6 givenname: R R surname: Friis fullname: Friis, R R |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16465217$$D View this record in MEDLINE/PubMed |
BookMark | eNpFUU1v2zAMFYYWS7-uOw467eaUsmzZ2S0o1q1AgV3asyFLVKLAljxTKbp_0Z88uQ2wA0GCfHwE37tkZyEGZOyLgLUA2d7SYW2sXVcVQFnCJ3YhqkYVdQXyLNeyhmIDVbNil0QHAFDNRn1mK6EqVZeiuWBv2ylOKZIn7gNPe-SzTnzU46jnv3w36GD5Ei8Y0qwHPs2Rkk74nVtMaJKPgUfHDQ5D7ui0LzRRND5DLN9hQOJH8mHHNTfRB-NtJirwdZqRaFk2Qwzv8ylTa7O_ZudOD4Q3p3zFnu9_PN39Kh5__3y42z4WRkqRir5RddWUti0luNpa1Yuy1SCcls3GmbYUEvpSOWc3uoW-1UrWCiQ6cKigcvKKffvgzWf_HJFSN3pa3tAB45G6LFTdCmgycP0BNPl1mtF10-wXdToB3eJBR4cue9CdPMgLX0_Mx35E-x9-El3-AyEjh-g |
CitedBy_id | crossref_primary_10_1006_mcne_2001_1092 crossref_primary_10_1038_sj_cdd_4401197 crossref_primary_10_1074_jbc_M509572200 crossref_primary_10_1016_j_mce_2006_02_013 crossref_primary_10_1016_j_nbd_2003_11_022 crossref_primary_10_1016_S0014_5793_97_01324_0 crossref_primary_10_1007_s13277_014_2956_z crossref_primary_10_1210_endo_140_10_7097 crossref_primary_10_1096_fasebj_12_14_1541 crossref_primary_10_1002_jnr_10461 crossref_primary_10_1071_RD15367 crossref_primary_10_1016_S1472_6483_10_60431_2 crossref_primary_10_1016_S0037_1963_01_90007_1 |
ContentType | Journal Article |
DBID | NPM AAYXX CITATION 7X8 |
DOI | 10.1038/sj.cdd.4400220 |
DatabaseName | PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | PubMed MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine Biology |
EISSN | 1476-5403 |
EndPage | 124 |
ExternalDocumentID | 10_1038_sj_cdd_4400220 16465217 |
Genre | Journal Article |
GroupedDBID | --- -Q- 0R~ 29B 2WC 36B 39C 3V. 4.4 406 53G 5GY 70F 7X7 88A 88E 8AO 8FE 8FH 8FI 8FJ 8R4 8R5 AACDK AAHBH AANZL AASDW AASML AATNV AAWBL AAZLF ABAKF ABAWZ ABDBF ABJNI ABLJU ABUWG ABZZP ACAOD ACGFS ACIWK ACKTT ACPRK ACRQY ACZOJ ADBBV ADFRT ADHDB AEFQL AEJRE AEMSY AENEX AEVLU AEXYK AFBBN AFKRA AFSHS AGAYW AGEZK AGHAI AGQEE AHMBA AHSBF AIGIU AILAN AJRNO ALFFA ALIPV ALMA_UNASSIGNED_HOLDINGS AMYLF AOIJS ASPBG AVWKF AXYYD AZFZN B0M BAWUL BBNVY BENPR BHPHI BKKNO BPHCQ BVXVI C1A CAG CCPQU COF CS3 DIK DNIVK DPUIP DU5 E3Z EAD EAP EBC EBD EBLON EBS EE. EIOEI EJD EMB EMK EMOBN EPL ESX F5P FDQFY FEDTE FERAY FIGPU FIZPM FSGXE FYUFA GX1 HCIFZ HMCUK HVGLF HYE HZ~ IWAJR JSO JZLTJ KQ8 L7B LK8 M0L M1P M7P NAO NPM NQJWS OK1 P2P PQQKQ PROAC PSQYO Q2X RIG RNS RNT RNTTT RPM SNX SNYQT SOHCF SRMVM SV3 SWTZT TAOOD TBHMF TDRGL TR2 TSG TUS UKHRP ~8M AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c331t-b765472d8230f5dd6b128a01fa379fc82130b26ffd9a80b8a635603ef0fe604f3 |
ISSN | 1350-9047 |
IngestDate | Sat Aug 17 03:46:54 EDT 2024 Thu Sep 26 17:10:30 EDT 2024 Sat Sep 28 07:45:58 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c331t-b765472d8230f5dd6b128a01fa379fc82130b26ffd9a80b8a635603ef0fe604f3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://www.nature.com/articles/4400220.pdf |
PMID | 16465217 |
PQID | 79658107 |
PQPubID | 23479 |
PageCount | 11 |
ParticipantIDs | proquest_miscellaneous_79658107 crossref_primary_10_1038_sj_cdd_4400220 pubmed_primary_16465217 |
PublicationCentury | 1900 |
PublicationDate | 1997-02-01 |
PublicationDateYYYYMMDD | 1997-02-01 |
PublicationDate_xml | – month: 02 year: 1997 text: 1997-02-01 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Cell death and differentiation |
PublicationTitleAlternate | Cell Death Differ |
PublicationYear | 1997 |
SSID | ssj0006796 |
Score | 1.644709 |
Snippet | Apoptosis plays a striking role in the hormone-dependent involution of the mammary gland, but it has proved difficult to distinguish between the 'cell death'... |
SourceID | proquest crossref pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 114 |
Title | Apoptosis in the rat mammary gland and ventral prostate: detection of cell death-associated genes using a coincident-expression cloning approach |
URI | https://www.ncbi.nlm.nih.gov/pubmed/16465217 https://search.proquest.com/docview/79658107 |
Volume | 4 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLbKJhAvCMatXP2AxEOUksRJnPDWTkwT0va0ib1FtmOzTltSrSnaeOMf8JM5vqUpbBLw0KhyKifK9_Xk8_G5IPROUCoyTuH_zSgPU8XzkCuhE4ETEUse8UzpbOSDw3z_OP18kp2MRj-G2SUdn4jvN-aV_A-qMAa46izZf0C2nxQG4DvgC0dAGI5_hfF00S66VpcUcdGKAGdwwUw6WmCac5i9gW_Wg6tjsWylCjINatlJ4dWi9t7DCIjBkDm4QId-1WYwWBlnAgtEq73yOq03lFcuerYJxLl15_rS5EOtu9vPau7Ct2Lp5hub_7O5PLcBQl_Wxt9469cq1dqjfg9ltjq9HPYLq10WH_Vxzr2dJVkUlpEttukNcTrgWzIwqrFNM_3D2NvS7suziajrSZpqORINfwhPc3FhoNdF1ECn0PVLrw9F9KfuoO2Elhms37ene7PZYf861442s2p3d-wrf5Liw-aldf1ZN9mmyLll5WIUzNFD9MAtPfDU8ugRGslmB921zUivd9C9Axdm8Rj97ImF5w0GYmEgFnbEwoZYWH8csbAn1kfc0wq3Cmta4d9phQ2tsKEVZvhGWmFHK-xp9QQd73062t0PXeuOUBASdyGnuql1UuttXJXVdc5BB7EoVozQUokiAenEk1ypumRFxAumyyRGRKpIyTxKFXmKtpq2kc8RVkVexqWUuUjqNJUJJwUstRjLKC9JKssxeu-fdbWwFVoqE1lBimp5VgFAlQNojN56KCowovohsEa2q2VFdQmkOKJj9MwitJ7JIfri1jMv0f01w1-hre5yJV-DUO34G0elX0Gsl7w |
link.rule.ids | 315,786,790,27957,27958 |
linkProvider | Library Specific Holdings |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Apoptosis+in+the+rat+mammary+gland+and+ventral+prostate%3A+detection+of+cell+death-associated+genes+using+a+coincident-expression+cloning+approach&rft.jtitle=Cell+death+and+differentiation&rft.au=Bielke%2C+W&rft.au=Ke%2C+G&rft.au=Feng%2C+Z&rft.au=Buhrer%2C+S&rft.date=1997-02-01&rft.issn=1350-9047&rft.volume=4&rft.issue=2&rft.spage=114&rft_id=info:doi/10.1038%2Fsj.cdd.4400220&rft_id=info%3Apmid%2F16465217&rft.externalDocID=16465217 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1350-9047&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1350-9047&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1350-9047&client=summon |