Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute (Corchorus capsularis L.)

• Published in: Journal of natural fibers Vol. 12; no. 4; pp. 303 - 310
• Main Authors: Zhang, Gaoyang, Zhang, Yujia, Xu, Jiantang, Li, Feng Tao, Tao, Aifen, Zhang, Liwu, Fang, Pingping, Lin, Lihui, Qi, Jianmin
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 04.07.2015
• Subjects: Acetosyringone; agar; Agrobacterium; coculture; Corchorus capsularis; cotyledonary node; culture media; explants; fiber crop; fiber quality; genes; jute; naphthaleneacetic acid; regeneration; rooting; shoots; sucrose; system optimization; Transformation

The purpose of this study was to use cotyledonary nodes as explants to establish an efficient regeneration protocol for jute (Corchorus capsularis L.) on Murashige and Skoog (MS) basal medium. Research into the different growth regulators using the orthogonal design L16 (4 ⁵) revealed that the best...