Detergent solubilization of human neutrophil leukotriene B4 receptors

• Published in: Biochemical and biophysical research communications Vol. 128; no. 2; pp. 878 - 883
• Main Authors: LIN, A. H, RUPPEL, P. L, GORMAN, R. R
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 30.04.1985
• Subjects: Applied sciences; Cholic Acids; Exact sciences and technology; Humans; Kinetics; Leukotriene B4 - metabolism; Neutrophils - analysis; Other techniques and industries; Receptors, Immunologic - blood; Receptors, Immunologic - isolation & purification; Receptors, Leukotriene B4; Solubility; Structure-Activity Relationship; Time Factors

Specific leukotriene B4 (LTB4) receptors in human neutrophils were solubilized by treatment of "receptor fraction" membranes with the zwitterionic detergent (3-[(3-cholamidopropyl)-dimethylammonio]1-propane sulfonate (CHAPS). The soluble receptors were assayed by polyethylene glycol (PEG)...