Ascorbate protects against tert-butyl hydroperoxide inhibition of erythrocyte membrane Ca2+ + Mg2+-ATPase

• Published in: Archives of biochemistry and biophysics Vol. 278; no. 2; pp. 416 - 424
• Main Authors: BLAINE MOORE, R, BAMBERG, A. D, WILSON, L. C, JENKINS, L. D, MANKAD, V. N
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 01.05.1990
• Subjects: Analytical, structural and metabolic biochemistry; Antioxidants - pharmacology; Ascorbic Acid - pharmacology; Biological and medical sciences; Ca(2+) Mg(2+)-ATPase - analysis; Ca(2+) Mg(2+)-ATPase - antagonists & inhibitors; Calcium-Transporting ATPases - analysis; Calcium-Transporting ATPases - antagonists & inhibitors; Cross-Linking Reagents - pharmacology; Enzymes and enzyme inhibitors; Erythrocyte Membrane - drug effects; Erythrocyte Membrane - enzymology; Fundamental and applied biological sciences. Psychology; Hemin - pharmacology; Hemoglobins - pharmacology; Humans; Hydrolases; Iron - pharmacology; Lipid Peroxidation; Malondialdehyde - analysis; Peroxides - pharmacology; Sulfhydryl Compounds - pharmacology; tert-Butylhydroperoxide; Red blood cell; Oxidation; Inhibition; Ca,Mg-ATPase; Plasma membrane; Hydrogen peroxides

The incubation of erythrocyte suspensions or isolated membranes containing a residual amount of hemoglobin (0.04% of original cellular hemoglobin) with tert-butyl hydroperoxide (tBHP, 0.5 mM) caused significant inhibition of basal and calmodulin-stimulated Ca2+ + Mg2(+)-ATPase activities and the for...