Regulatory Properties of an Inorganic Pyrophosphatase from the Photosynthetic Bacterium Rhodospirillum rubrum

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 68; no. 4; pp. 721 - 725
• Main Authors: Klemme, Jobst-Heinrich, Gest, Howard
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences of the United States of America 01.04.1971; National Acad Sciences
• Subjects: Biological Sciences: Biochemistry; Biosynthesis; Chromatophores; Enzymes; Kinetics; Molecular weight; Nucleotides; Ratios; Reaction kinetics; Reagents; Sulfates

In Rhodospirillum rubrum, inorganic pyrophosphatase activity is observed in both the cytoplasmic and membrane fractions. The soluble enzyme accounts for about 80% of the total activity in crude extracts, and is the subject of this report. Zn2+is required for both activity and stability of the enzyme...