Development and Validation of an LC-MS/MS-ESI Method for Comparative Pharmacokinetic Study of Ciprofloxacin in Healthy Male Subjects

• Published in: Drug research Vol. 67; no. 2; p. 94
• Main Authors: Choudhury, Hira, Gorain, Bapi, Paul, Anwesha, Sarkar, Pradipta, Dan, Shubhasis, Chakraborty, Pragnya, Pal, Tapan K
• Format: Journal Article
• Language: English
• Published: Germany 01.02.2017
• Subjects: Calibration; Chromatography, Liquid - methods; Chromatography, Liquid - standards; Ciprofloxacin - blood; Ciprofloxacin - pharmacokinetics; Cross-Over Studies; Drug Stability; Humans; Liquid-Liquid Extraction; Male; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Spectrometry, Mass, Electrospray Ionization - standards; Tandem Mass Spectrometry - methods; Tandem Mass Spectrometry - standards; Therapeutic Equivalency

A sensitive, specific and reproducible liquid chromatography coupled to tandem mass spectrometric method was developed and validated for the estimation of ciprofloxacin, an extensively used second-generation quinolone antibiotics, in human plasma. A liquid-liquid extraction of ciprofloxacin and the...