A rapid lateral flow assay using immunomagnetic nanoparticles for detecting mango allergen residues in processed foods

• Published in: Journal of food safety Vol. 41; no. 6
• Main Authors: Tsai, Wen‐Che, Yin, Hsin‐Yi, Lin, Ya‐Ting, Liu, Hung‐Jen, Tseng, Cheng‐Yen, Wen, Hsiao‐Wei
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.12.2021; Blackwell Publishers Inc
• Subjects: Allergens; Assaying; detection limit; Food; Food allergies; Food processing; Food products; Food safety; Mangoes; Monoclonal antibodies; Nanoparticles; Optimization; Processed foods

This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products. Monoclonal antibodies (2F1 and 21A5) were prepared; mAb‐2F1 was modified on the surface of IMNPs and mAb‐21A5 was immobilized on the test line...