A rapid lateral flow assay using immunomagnetic nanoparticles for detecting mango allergen residues in processed foods

This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products. Monoclonal antibodies (2F1 and 21A5) were prepared; mAb‐2F1 was modified on the surface of IMNPs and mAb‐21A5 was immobilized on the test line...

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Published inJournal of food safety Vol. 41; no. 6
Main Authors Tsai, Wen‐Che, Yin, Hsin‐Yi, Lin, Ya‐Ting, Liu, Hung‐Jen, Tseng, Cheng‐Yen, Wen, Hsiao‐Wei
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.12.2021
Blackwell Publishers Inc
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Abstract This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products. Monoclonal antibodies (2F1 and 21A5) were prepared; mAb‐2F1 was modified on the surface of IMNPs and mAb‐21A5 was immobilized on the test line of the LFA strips. Following optimization, the limit of detection (LOD) of this developed LFA was 6.2 ng/mL for rMan i1 with a linear range of 5–100 ng/mL. The assay also had high specificity in the analysis of nine vegetables and fruits. Furthermore, 22 food products were analyzed by this assay and mango allergens were detected in 13 food products that contained mango. In conclusion, a rapid LFA that uses IMNPs was developed herein for the detection of the major mango allergen Man i1 in processed foods. The rapid detection method of IMNP‐based LFA is developed for detecting mango major allergen (Man i1).
AbstractList This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products. Monoclonal antibodies (2F1 and 21A5) were prepared; mAb‐2F1 was modified on the surface of IMNPs and mAb‐21A5 was immobilized on the test line of the LFA strips. Following optimization, the limit of detection (LOD) of this developed LFA was 6.2 ng/mL for rMan i1 with a linear range of 5–100 ng/mL. The assay also had high specificity in the analysis of nine vegetables and fruits. Furthermore, 22 food products were analyzed by this assay and mango allergens were detected in 13 food products that contained mango. In conclusion, a rapid LFA that uses IMNPs was developed herein for the detection of the major mango allergen Man i1 in processed foods.
This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products. Monoclonal antibodies (2F1 and 21A5) were prepared; mAb‐2F1 was modified on the surface of IMNPs and mAb‐21A5 was immobilized on the test line of the LFA strips. Following optimization, the limit of detection (LOD) of this developed LFA was 6.2 ng/mL for rMan i1 with a linear range of 5–100 ng/mL. The assay also had high specificity in the analysis of nine vegetables and fruits. Furthermore, 22 food products were analyzed by this assay and mango allergens were detected in 13 food products that contained mango. In conclusion, a rapid LFA that uses IMNPs was developed herein for the detection of the major mango allergen Man i1 in processed foods. The rapid detection method of IMNP‐based LFA is developed for detecting mango major allergen (Man i1).
This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products. Monoclonal antibodies (2F1 and 21A5) were prepared; mAb‐2F1 was modified on the surface of IMNPs and mAb‐21A5 was immobilized on the test line of the LFA strips. Following optimization, the limit of detection (LOD) of this developed LFA was 6.2 ng/mL for rMan i1 with a linear range of 5–100 ng/mL. The assay also had high specificity in the analysis of nine vegetables and fruits. Furthermore, 22 food products were analyzed by this assay and mango allergens were detected in 13 food products that contained mango. In conclusion, a rapid LFA that uses IMNPs was developed herein for the detection of the major mango allergen Man i1 in processed foods.
Author Tsai, Wen‐Che
Yin, Hsin‐Yi
Lin, Ya‐Ting
Liu, Hung‐Jen
Wen, Hsiao‐Wei
Tseng, Cheng‐Yen
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Snippet This study develops a rapid lateral flow assay (LFA) that uses immunomagnetic nanoparticles (IMNPs) to detect mango major allergen (Man i1) in food products....
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wiley
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SubjectTerms Allergens
Assaying
detection limit
Food
Food allergies
Food processing
Food products
Food safety
Mangoes
Monoclonal antibodies
Nanoparticles
Optimization
Processed foods
Title A rapid lateral flow assay using immunomagnetic nanoparticles for detecting mango allergen residues in processed foods
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fjfs.12929
https://www.proquest.com/docview/2608289959
https://www.proquest.com/docview/2636402450
Volume 41
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