Anionic peroxidase‐mediated oxidative burst requirement for jasmonic acid‐dependent Solanum tuberosum defence against Phytophthora infestans

• Published in: Plant pathology Vol. 67; no. 2; pp. 349 - 357
• Main Authors: Sorokan, A. V., Burhanova, G. F., Maksimov, I. V.
• Format: Journal Article
• Language: English
• Published: Oxford Wiley Subscription Services, Inc 01.02.2018
• Subjects: Acid resistance; anionic peroxidase; Antagonists; Antisense RNA; Biosynthesis; Cell walls; Deposition; Gene expression; genes; Herbivores; Hydrogen peroxide; Immunity; Jasmonic acid; Lignin; NAD(P)H oxidase; NADP (coenzyme); Pathogens; Penetration resistance; Peroxidase; Phytophthora infestans; Plants (botany); Reactive oxygen species; salicylic acid; salicylic acids; Solanum tuberosum; Transcription; transcription (genetics)
• ISSN: 0032-0862; 1365-3059
• DOI: 10.1111/ppa.12743

Plants are subject to pervasive attack by a diversity of pathogens and herbivores. It is considered that salicylic (SA) and jasmonic (JA) acids induce systemic resistance to these antagonists. Different lines of evidence indicate that the interplay of SA and JA with reactive oxygen species (ROS), generated by NADPH oxidases or apoplastic peroxidases, has an important role in the plant defence response. It was previously shown that diminished expression of the Solanum tuberosum anionic peroxidase gene M21334, as a consequence of antisense expression (asAPO plants), results in high susceptibility to Phytophthora infestans and deficient lignin deposition in cell walls. Further research reported in this paper showed that asAPO antisense plants have a low level of H2O2 and impaired JA‐associated responses, such as reduced transcription of JA‐responsive and JA‐biosynthesis genes. The asAPO plants did not exhibit any apparent defect in their susceptibility to SA. Exogenous H2O2 partially restored JA‐dependent reactions in asAPO plants, together with an increase in lignin deposition around pathogen‐penetrated sites, resulting in increased resistance of asAPO to P. infestans. These data demonstrate that the anionic peroxidase‐dependent oxidative burst plays an important role in the elicitation of JA‐associated immunity.