Defining the requirements for the conjugative transfer of Rhizobium leguminosarum plasmid pRleVF39b
strain VF39 contains a plasmid, pRleVF39b, which encodes a distinctive type of conjugation system (rhizobial type IVa) that is relatively widespread among rhizobial genomes. The cluster of genes encoding the transfer functions lacks orthologs to genes such as , and , but contains 15 conserved genes...
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| Published in | Microbiology (Society for General Microbiology) Vol. 166; no. 3; pp. 318 - 331 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.03.2020
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| Abstract | strain VF39 contains a plasmid, pRleVF39b, which encodes a distinctive type of conjugation system (rhizobial type IVa) that is relatively widespread among rhizobial genomes. The cluster of genes encoding the transfer functions lacks orthologs to genes such as
,
and
, but contains 15 conserved genes of unknown function. We determined the importance of these genes in conjugation by constructing marked and unmarked mutations in each gene, and established that six genes, now designated
, played a significant role in plasmid transfer. Like the relaxase gene,
, and the genes encoding the MPF system (
genes), five of these genes, located in two divergently transcribed operons, are regulated by the Xre family repressor TrbR. The other gene,
encodes a protein with similarity to histidinol phosphatases, and its role in conjugation is unclear, but mutations in
are severely impaired for conjugation. TrcF does not play a role in regulation of other conjugation genes. |
|---|---|
| AbstractList | Rhizobium leguminosarum
strain VF39 contains a plasmid, pRleVF39b, which encodes a distinctive type of conjugation system (rhizobial type IVa) that is relatively widespread among rhizobial genomes. The cluster of genes encoding the transfer functions lacks orthologs to genes such as
traCD
,
traF
and
traB
, but contains 15 conserved genes of unknown function. We determined the importance of these genes in conjugation by constructing marked and unmarked mutations in each gene, and established that six genes, now designated
trcA-F
, played a significant role in plasmid transfer. Like the relaxase gene,
traA
, and the genes encoding the MPF system (
trb
genes), five of these genes, located in two divergently transcribed operons, are regulated by the Xre family repressor TrbR. The other gene,
trcF
encodes a protein with similarity to histidinol phosphatases, and its role in conjugation is unclear, but mutations in
trcF
are severely impaired for conjugation. TrcF does not play a role in regulation of other conjugation genes. strain VF39 contains a plasmid, pRleVF39b, which encodes a distinctive type of conjugation system (rhizobial type IVa) that is relatively widespread among rhizobial genomes. The cluster of genes encoding the transfer functions lacks orthologs to genes such as , and , but contains 15 conserved genes of unknown function. We determined the importance of these genes in conjugation by constructing marked and unmarked mutations in each gene, and established that six genes, now designated , played a significant role in plasmid transfer. Like the relaxase gene, , and the genes encoding the MPF system ( genes), five of these genes, located in two divergently transcribed operons, are regulated by the Xre family repressor TrbR. The other gene, encodes a protein with similarity to histidinol phosphatases, and its role in conjugation is unclear, but mutations in are severely impaired for conjugation. TrcF does not play a role in regulation of other conjugation genes. |
| Author | Hynes, Michael F Wathugala, N Dulmini Hemananda, Kasuni M Yip, Cynthia B |
| Author_xml | – sequence: 1 givenname: N Dulmini surname: Wathugala fullname: Wathugala, N Dulmini organization: Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada – sequence: 2 givenname: Kasuni M surname: Hemananda fullname: Hemananda, Kasuni M organization: Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada – sequence: 3 givenname: Cynthia B surname: Yip fullname: Yip, Cynthia B organization: Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada – sequence: 4 givenname: Michael F surname: Hynes fullname: Hynes, Michael F organization: Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31935189$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/0378-1119(95)00584-1 10.1016/j.plasmid.2011.12.010 10.1016/0147-619X(78)90016-1 10.1099/00221287-148-2-615 10.1128/MMBR.00020-10 10.1016/0378-1119(93)90611-6 10.1099/13500872-145-6-1307 10.3390/genes10080601 10.1073/pnas.0908978107 10.1007/BF00408302 10.1128/JB.01234-12 10.1186/1471-2180-10-219 10.1128/JB.183.7.2394-2397.2001 10.1016/0378-1119(95)00108-I 10.1073/pnas.83.22.8447 10.1111/j.1574-6968.2009.01556.x 10.1016/j.plasmid.2017.04.002 10.1016/S0022-2836(05)80360-2 10.1111/j.1365-2958.1990.tb00625.x 10.1016/0378-1119(87)90041-2 10.1038/sj.emboj.7601696 10.1111/1462-2920.14645 10.1007/s13199-017-0476-5 10.1099/mic.0.28938-0 10.1128/JB.186.22.7538-7548.2004 10.2478/s11535-012-0058-8 10.1128/JB.183.6.2141-2144.2001 10.1007/s00203-004-0736-7 10.2135/cropsci2011.08.0459 10.1038/nbt1183-784 10.1128/jb.171.11.6161-6168.1989 10.1007/s004380050582 10.1099/mic.0.28311-0 10.3389/fmicb.2014.00793 10.1139/W09-056 10.1016/0147-619X(85)90062-9 10.1111/1574-6941.12325 10.1099/00221287-84-1-188 10.1128/JB.00701-06 10.1111/mmi.12079 |
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| References | Thoma (R41) 2009; 94 Sambrook (R16) 1989 Singh (R17) 2012; 52 R40 R43 R20 R42 R23 R22 R25 R24 R27 R26 R29 R28 R1 R2 R3 R4 R5 R6 R7 R8 R9 Yip (R21) 2013 R30 R10 R32 R31 R12 R34 R11 R33 R36 R13 Ding (R14) 2012 R35 R38 R15 R37 R18 R39 R19 |
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| Snippet | strain VF39 contains a plasmid, pRleVF39b, which encodes a distinctive type of conjugation system (rhizobial type IVa) that is relatively widespread among... Rhizobium leguminosarum strain VF39 contains a plasmid, pRleVF39b, which encodes a distinctive type of conjugation system (rhizobial type IVa) that is... |
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| Title | Defining the requirements for the conjugative transfer of Rhizobium leguminosarum plasmid pRleVF39b |
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