Bovine Mammary Gland Biopsy Techniques

• Published in: Journal of visualized experiments no. 142
• Main Authors: Daley, Veridiana L., Dye, Charles, Bogers, Sophie H., Akers, R. Michael, Rodriguez, Francisco C., Cant, John P., Doelman, John, Yoder, Peter, Kumar, Karan, Webster, Dane, Hanigan, Mark D.
• Format: Journal Article
• Language: English
• Published: United States 23.12.2018
• Subjects: Animals; aseptic conditions; biopsy; Biopsy - methods; Biopsy - veterinary; body weight; Cattle; cell culture; dairy cows; Female; flunixin; gene expression; hemorrhage; hemostasis; histology; Holstein; intravenous injection; lidocaine; mammary glands; Mammary Glands, Animal - pathology; Mammary Glands, Animal - surgery; milk yield; risk; sedation; signal transduction; stainless steel; subcutaneous injection; translation (genetics); xylazine

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dai...