Label-free and sensitive detection of uracil-DNA glycosylase using exponential real-time rolling circle amplification

• Published in: Analytical methods Vol. 10; no. 20; pp. 2405 - 2410
• Main Authors: Xu, Yuan, Cui, Yun-Xi, Zhao, Qiu-Ge, Tang, An-Na, Kong, De-Ming
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 01.01.2018
• Subjects: Amplification; analytical methods; biochemical pathways; Contamination; Deoxyribonucleic acid; DNA; DNA glycosylase; Endonuclease; Feasibility studies; Fluorescence; Nicking endonuclease; Oligonucleotides; prognosis; Real time; risk reduction; Strategy; Uracil; Uracil-DNA glycosidase; uracil-DNA glycosylase

Sensitive evaluation of the uracil-DNA glycosylase (UDG) activity is greatly significant in both fundamental biochemical process studies and disease prognosis. In this study, a simple but sensitive UDG activity-sensing strategy was designed on the basis of UDG-triggered rolling circle amplification...