Morphological and molecular evidence reveals a new species of Lyperosomum Looss, 1899 (Digenea: Dicrocoeliidae) from Melanerpes aurifrons (Wagler, 1829) from northern Mexico

• Published in: Journal of helminthology Vol. 94; p. e156
• Main Authors: González-García, M T, Ortega-Olivares, M P, Andrade-Gómez, L, García-Varela, M
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press 01.01.2020
• Subjects: Animals; Bayes Theorem; Bayesian analysis; Birds - parasitology; Bladder; Body length; Central America; Cytochrome; Cytochrome-c oxidase; Cytochromes; Datasets; Deoxyribonucleic acid; Dicrocoeliidae - anatomy & histology; Dicrocoeliidae - classification; Divergence; DNA; DNA, Helminth - genetics; DNA, Mitochondrial - genetics; DNA, Ribosomal - genetics; Female; Genetic Variation; Intestine; Intestines; Lyperosomum; Mammals; Melanerpes aurifrons; Mexico; Mitochondrial DNA; Morphology; New records; New species; Nucleotide sequence; Parasites; Phylogenetics; Phylogeny; Physical characteristics; Probability theory; Ribosomal DNA; Sequence Analysis, DNA; Taxonomy; Worms; Zonorchis; Central America; Mexico; Mississippi; Czech Republic; United States > US; China; Texas; Ukraine; Poland; Uganda; Georgia; Florida; Costa Rica; Lyperosomum; Morphology; molecular phylogeny; Mexico; cox 1; taxonomy; LSU
• ISSN: 0022-149X; 1475-2697
• DOI: 10.1017/S0022149X20000425

A new species of the genus Lyperosomum Looss, 1899, from the intestine of the golden-fronted woodpecker (Melanerpes aurifrons) from northern Mexico is described. Lyperosomum cuauhxinqui sp. n. is morphologically distinguished from other congeneric species from the Americas by a higher oral/ventral sucker ratio and its body length and width. The sequences of domains D1-D3 of the large subunit (LSU) of nuclear ribosomal DNA and cytochrome c oxidase subunit 1 (cox 1) from the mitochondrial DNA of the new species were obtained and compared with available sequences from GenBank. The genetic divergence estimated between the new species and other congeneric species ranged from 2 to 6% and 13.4 to 17.3% for LSU and cox 1, respectively. Phylogenetic analyses based on the two (LSU and cox 1) molecular markers consistently showed that L. cuauhxinqui sp. n. was nested within the genus Lyperosomum, with strong bootstrap support (100%) and Bayesian posterior probabilities (1.0). In particular, the LSU tree indicated that the sequence of the new species is closely related to sequences from Zonorchis alveyi, Zonorchis delectans and Zonorchis sp. from Central America, suggesting that these sequences should be transferred to the genus Lyperosomum. The new species represents the first record from Mexico and the fifth species identified in the Americas. Our study also revealed that the taxonomy of the genus Lyperosomum should be re-examined by combining molecular, morphological and ecological characteristics.