The problem of hypernegative supercoiling and r-loop formation in transcription

• Published in: Frontiers in bioscience Vol. 8; no. 4; pp. d210 - 221
• Main Author: Drolet, Marc
• Format: Journal Article
• Language: English
• Published: United States 2003
• Subjects: DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Superhelical - chemistry; DNA, Superhelical - genetics; Nucleic Acid Conformation; Transcription, Genetic

DNA supercoiling and topoisomerases have long been known to affect transcription initiation. In many studies, topA mutants were used to perturb chromosomal supercoiling. Although such studies clearly revealed that supercoiling could significantly affect gene expression, they did not tell much about...