Droplet-free digital immunoassay based on electrochemiluminescence

• Published in: Biosensors and bioelectronics. X Vol. 13; p. 100312
• Main Authors: Ito, Kentaro, Inoue, Kumi Y., Ino, Kosuke, Shiku, Hitoshi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2023; Elsevier
• Subjects: Digital immunoassay; Electrochemiluminescence; Electrochemistry; Tyramide signal amplification; Electrochemistry; Electrochemiluminescence; Tyramide signal amplification; Digital immunoassay

Here, we report a droplet-free digital immunoassay for goat immunoglobulin G (IgG) based on electrochemiluminescence (ECL) for simplified digital immunoassay. Digital enzyme-linked immunosorbent assay (ELISA) enabled ultrasensitive detection using digital counting immuno-reaction-induced femtoliter-...