Cross-Linking of T Cell Mitogens: Effects on B and T Cell Proliferation and Immunoglobulin Synthesis

The T cell mitogens Pa-2, concanavalin A (con A) and its dimeric derivative succinyl-con A, were each cross-linked with the bifunctional reagent dimethyl suberimidate. Although the dose-response curves of these insoluble aggregated products were markedly changed from those of the soluble mitogens, e...

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Bibliographic Details
Published inThe Journal of immunology (1950) Vol. 118; no. 3; pp. 863 - 868
Main Authors Basham, T. Y, Waxdal, M. J
Format Journal Article
LanguageEnglish
Published United States Am Assoc Immnol 01.03.1977
Subjects
Animals
Antibody Formation
B-Lymphocytes - immunology
Concanavalin A - analogs & derivatives
Concanavalin A - pharmacology
DNA Replication
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, Nude
Mitogens
Molecular Conformation
T-Lymphocytes - immunology
Thymidine - metabolism
Tritium
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Summary:The T cell mitogens Pa-2, concanavalin A (con A) and its dimeric derivative succinyl-con A, were each cross-linked with the bifunctional reagent dimethyl suberimidate. Although the dose-response curves of these insoluble aggregated products were markedly changed from those of the soluble mitogens, each aggregate continued to stimulate DNA synthesis by murine thymus and T cells. Both aggregated Pa-2 and aggregated succinyl con A stimulated DNA synthesis by B cells from athymic (Nu/Nu) mice. Aggregated con A did not stimulate these cells and, like soluble con A, depressed the background incorporation of 3H-thymidine. Unlike soluble Pa-2, aggregated Pa-2 also greatly increased Ig production by both the B cell cultures and B + T cell cultures from normal (BALB/c) mice.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.118.3.863