Preliminary investigations on a glucose biosensor based on the potentiometric principle
In this study, an electron mediator and a simple immobilization process are adopted to fabricate a potentiometric glucose biosensor. The enzyme and the electron mediator are immobilized on the surface of tin oxide (SnO 2)/indium tin oxide (ITO) glass using a covalent bond method to develop a disposa...
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| Published in | Sensors and actuators. B, Chemical Vol. 123; no. 2; pp. 720 - 726 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
21.05.2007
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| Subjects | |
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| Abstract | In this study, an electron mediator and a simple immobilization process are adopted to fabricate a potentiometric glucose biosensor. The enzyme and the electron mediator are immobilized on the surface of tin oxide (SnO
2)/indium tin oxide (ITO) glass using a covalent bond method to develop a disposable potentiometric glucose biosensor. The SnO
2/ITO glass is a pH sensor fabricated by depositing SnO
2 thin films onto an ITO glass. The glucose oxidase (GOD) and the electron mediator (ferrocenecarboxylic acid, FcA) are co-immobilized on the SnO
2/ITO glass using 3-glycidyloxypropyltrimethoxysilane (GPTS). This work investigates the coimmobilization of GOD and FcA as a useful approach for enlarging the dynamic range to a glucose concentration of 360
mg/dl, and for improving linearity and sensitivity of the fabricated glucose biosensor. The experimental results indicate that the optimal weight ratio of GOD to FcA is 1:1. The output signal is associated with the pH of the measurement environment and the optimal pH value is pH 7.5. |
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| AbstractList | In this study, an electron mediator and a simple immobilization process are adopted to fabricate a potentiometric glucose biosensor. The enzyme and the electron mediator are immobilized on the surface of tin oxide (SnO
2)/indium tin oxide (ITO) glass using a covalent bond method to develop a disposable potentiometric glucose biosensor. The SnO
2/ITO glass is a pH sensor fabricated by depositing SnO
2 thin films onto an ITO glass. The glucose oxidase (GOD) and the electron mediator (ferrocenecarboxylic acid, FcA) are co-immobilized on the SnO
2/ITO glass using 3-glycidyloxypropyltrimethoxysilane (GPTS). This work investigates the coimmobilization of GOD and FcA as a useful approach for enlarging the dynamic range to a glucose concentration of 360
mg/dl, and for improving linearity and sensitivity of the fabricated glucose biosensor. The experimental results indicate that the optimal weight ratio of GOD to FcA is 1:1. The output signal is associated with the pH of the measurement environment and the optimal pH value is pH 7.5. In this study, an electron mediator and a simple immobilization process are adopted to fabricate a potentiometric glucose biosensor. The enzyme and the electron mediator are immobilized on the surface of tin oxide (SnO sub(2))/indium tin oxide (ITO) glass using a covalent bond method to develop a disposable potentiometric glucose biosensor. The SnO sub(2)/ITO glass is a pH sensor fabricated by depositing SnO sub(2) thin films onto an ITO glass. The glucose oxidase (GOD) and the electron mediator (ferrocenecarboxylic acid, FcA) are co-immobilized on the SnO sub(2)/ITO glass using 3-glycidyloxypropyltrimethoxysilane (GPTS). This work investigates the coimmobilization of GOD and FcA as a useful approach for enlarging the dynamic range to a glucose concentration of 360mg/dl, and for improving linearity and sensitivity of the fabricated glucose biosensor. The experimental results indicate that the optimal weight ratio of GOD to FcA is 1:1. The output signal is associated with the pH of the measurement environment and the optimal pH value is pH 7.5. |
| Author | Hsiung, Shen-Kan Chou, Jung-Chuan Sun, Tai-Ping Liao, Cheng-Wei Hsieh, Jui-Hsiang |
| Author_xml | – sequence: 1 givenname: Cheng-Wei surname: Liao fullname: Liao, Cheng-Wei organization: Institute of Biomedical Engineering, Chung Yuan Christian University, Chung-Li 320, Taiwan, ROC – sequence: 2 givenname: Jung-Chuan surname: Chou fullname: Chou, Jung-Chuan email: choujc@yuntech.edu.tw organization: Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, 123, Sec.3, University Rd., Douliou, Yunlin 640, Taiwan, ROC – sequence: 3 givenname: Tai-Ping surname: Sun fullname: Sun, Tai-Ping organization: Institute of Electrical Engineering, National Chi Nan University, Nantou 545, Taiwan, ROC – sequence: 4 givenname: Shen-Kan surname: Hsiung fullname: Hsiung, Shen-Kan organization: Institute of Electronic Engineering, Chung Yuan Christian University, Chung-Li 320, Taiwan, ROC – sequence: 5 givenname: Jui-Hsiang surname: Hsieh fullname: Hsieh, Jui-Hsiang organization: Institute of Biomedical Engineering, Chung Yuan Christian University, Chung-Li 320, Taiwan, ROC |
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| Keywords | Electron mediator 3-Glycidyloxypropyltrimethoxysilane (GPTS) Tin oxide (SnO 2) Glucose oxidase (GOD) Potentiometric biosensor |
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| SubjectTerms | 3-Glycidyloxypropyltrimethoxysilane (GPTS) Electron mediator Glucose oxidase (GOD) Potentiometric biosensor Tin oxide (SnO 2) |
| Title | Preliminary investigations on a glucose biosensor based on the potentiometric principle |
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